PESTICINS I

Journal of Bacteriology - Tập 82 Số 6 - Trang 940-949 - 1961
Robert R. Brubaker1, Michael J. Surgalla1
1U. S. Army Biological Laboratories, Fort Detrick, Frederick, Maryland

Tóm tắt

Brubaker, Robert R. (U. S. Army Biological Laboratories, Fort Detrick, Frederick, Md.) and Michael J. Surgalla . Pesticins. I. Pesticin-bacterium interrelationships, and environmental factors influencing activity. J. Bacteriol. 82: 940–949. 1961—A second bacteriocin-like substance produced by all tested strains of Pasteurella pestis and P. pseudotuberculosis is described. This activity, termed pesticin II, is active against the two avirulent P. pestis strains, A12 and Java. These strains do not produce pesticin I, which inhibits the growth of type I strains of P. pseudotuberculosis . Pesticin I was also found to be active against certain strains of Escherichia coli , strain A12, but not strain Java, and some P. pestis isolates which also produce pesticin I. A number of E. coli strains produce a substance which also inhibits the growth of strains A12 and Java; the activity of this substance is dependent upon the presence of high concentrations of Ca ++ . The activity of both pesticins is inhibited under anaerobic conditions or in the presence of antiserum. The activity of pesticin I, but not pesticin II, is suppressed by Fe +++ , hemin, certain hemin-containing proteins, Mg ++ , and inorganic phosphate. Suppression of pesticin I activity by Fe +++ can be reversed by the addition of either Ca ++ or Sr ++ or by metal chelating agents. All tested strains of P. pestis and P. pseudotuberculosis produce a metabolite which suppresses the activity of pesticin I. The activity of this substance, termed pesticin I inhibitor, is enhanced by Fe +++ , and to a lesser extent by Mg ++ or by inorganic phosphate; its activity is suppressed by Mn ++ and by protamine sulfate.

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Tài liệu tham khảo

BEN-GURION , R. , AND I. HERTMAN. 1958 . Bacteriocin-like material produced by Pasteurella pestis . J. Gen. Microbiol. 19 : 289 - 297 .

BORDET P. 1948. Inhibition de l'action d'antibiotiques par les serums antibacteriens. Compt. rend. soc. biol. 142:257-259.

BURROWS , T. W. , AND G. A. BACON. 1956 . The basis of virulence in Pasteurella pestis: an antigen determining virulence . Brit. J. Exptl. Pathol. , 37 : 481 - 493 .

BURROWS , T. W. , AND G. A. BACON. 1960 . V and W antigens in strains of Pasteurella pseudotuberculosis . Brit. J. Exptl. Pathol. 39 : 278 - 291 .

FREDERICQ P. 1948. Sur la destruction des colicines par les proteases microbiennes. Compt. rend. soc. biol. 142:403-405.

FREDERICQ P. 1956. Resistance et immunite aux colicines. Compt. rend. soc. biol. 150:1514-1517.

FREDERICQ P. 1958. Colicines and colicinogenic factors p. 104-122. In Symposia of the Society for Experimental Biology. Academic Press Inc. New York N. Y.

GARBER , E. D. , K. NOBEL , A ND N . CAROUSO. 1953 . Genetic studies on the development of streptomycin resistance in Pasteurella pestis . J. Bacteriol. 65 : 485 - 489 .

HERTMAN , I. , AND R. BEN-GURION . 1958 . A study of pesticin bio-synthesis . J. Gen. Microbiol. 21 : 135 - 143 .

HIGUCHI , K., L. L. KUPFERBERG , A ND J . L. SMITH. 1959 . Studies on the nutrition and physiology of P. pestis. III. Effects of calcium ions on the growth of virulent and avirulent strains of Pasteurella pestis . J. Bacteriol. 77 : 317 - 321 .

HIGUCHI , K. , AND J. L. SMITH. 1961 . Studies on the nutrition and physiology of Pasteurella pestis. VI. A differential plating medium for the estimation of the mutation rate to avirulence . J. Bacteriol. 81 : 605 - 608 .

JACKSON , S. , AND T. W. BURROWS. 1956 . The virulence enhancing effect of iron on non-pigmented mutants of virulent strains of Pasteurella pestis . Brit. J. Exptl. Pathol. 37 : 577 - 583 .

JACOB F. AND E. L. WOLLMAN. 1958. Les episomes elements genetiques ajoutes. Compt. rend. 247:154-156.

LINTON , A. H. 1958 . Influence of inoculum size on antibiotic assays by agar diffusion technique with Klebsiella pneumoniae and streptomycin . J. Bacteriol. 76 : 94 - 103 .

MEYER K. F. 1958. Pasteurella p. 400-436. In R. Dubois [ed.] Bacterial and mycotic infections of man J. B. Lippincott Co. Philadelphia Pa.

OGG , J. E. , S. B. FRIEDMAN , A. W. ANDREWS , A ND M . J. SURGALLA. 1958 . Factors influencing the loss of virulence in Pasteurella pestis . J. Bacteriol. 76 : 185 - 191 .

RYAN , F. J. , P. FRIED , A ND K . MUKAI. 1955 . A colicin produced by cells which are sensitive to it . Biochem. et Biophys. Acta 18 : 131 .

SURGALLA , M. J. 1960 . Properties of virulent and avirulent strains of Pasteurella pestis . Ann. N. Y. Acad. Sci. 88 : 1136 - 1145 .

THAL E. 1954. Untersuchungen uber Pasteurella pseudotuberculosis unter besonderer Berucksichtigung ihres immunologischen Verhaltens. Lund Berlingska Boktryckeriet.

WESSMAN , G. E. , D. J. MILLER , A ND M . J. SURGALLA. 1958 . Toxic effect of glucose on virulent Pasteurella pestis in chemically defined media . J. Bacteriol. 76 : 368 - 375 .

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Journal of Bacteriology

Tập 82 Số 6

940-949

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