Overexpression of the Wilms' tumor gene WT1 in pancreatic ductal adenocarcinoma

Cancer Science - Tập 95 Số 7 - Trang 583-587 - 2004
Yusuke Oji1, Shoji Nakamori2, Masahiro Fujikawa3, Shin‐ichi Nakatsuka4, Asumi Yokota1, N Tatsumi1, Sakie Abeno1, Ai Ikeba1, S Takashima1, Masanori Tsujie2, Hirofumi Yamamoto2, Masato Sakon2, Riichiro Nezu3, Kiyoshi Kawano5, Sumiyuki Nishida6, Kazuhiro Ikegame7, Manabu Kawakami7, Akihiro Tsuboi7, Yoshihiro Oka6, Kiyoshi Yoshikawa3, Katsuyuki Aozasa4, Morito Monden2, Haruo Sugiyama1
1Department of Functional Diagnostic Science, Osaka University Graduate School of Medicine, 1-7 Yamada-Oka, Suita, Osaka 565-0871
2Department of Surgery and Clinical Oncology, Osaka University Graduate School of Medicine, 2-2 Yamada-Oka, Suita, Osaka 565-0871
3Department of Surgery, Osaka Rosai Hospital, 1179-3 Nagasone-cho, Sakai, Osaka 591-8025
4Department of Pathology, Osaka University Graduate School of Medicine, 2-2 Yamada-Oka, Suita, Osaka 565-0871
5Department of Pathology, Osaka Rosai Hospital, 1179-3 Nagasone-cho, Sakai, Osaka 591-8025
6Department of Molecular Medicine, Osaka University Graduate School of Medicine, 2-2 Yamada-Oka, Suita, Osaka 565-0871
7Department of Cancer Immunotherapy, Osaka University Graduate School of Medicine, 2-2 Yamada-Oka, Suita, Osaka 565-0871

Tóm tắt

The expression of the Wilms' tumor gene WT1 was examined by immunohistochemistry in 40 cases of pancreatic ductal adenocarcinoma. WT1 protein was expressed in 30 (75%) of the 40 pancreatic ductal adenocarcinomas, but not in the remaining 10 (25%). In normal pancreatic ductal cells, WT1 protein was undetectable. No correlations between WT1 expression and clinicopathological parameters such as age, sex, T or N stage, tumor location, and tumor differentiation were observed. Treatment with WT1 anti‐sense oligomers significantly inhibited the growth of five human pancreatic cancer cell lines, PSN1, MiaPaCa2, ASPC1, BxPC3, and PCI6, expressing the WT1 gene. These results indicate an important role of the WT1 gene in the tumorigenesis of pancreatic ductal adenocarcinoma expressing WT1 and provide a rationale for new treatment strategies to treat pancreatic ductal adenocarcinoma by targeting the WT1 gene and its product.

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Cancer Science

Tập 95 Số 7

583-587

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