Ouabain-resistant mutants of Chinese hamster ovary cells are not directly affected in Na+, K+-ATPase

Springer Science and Business Media LLC - Tập 12 - Trang 367-373 - 1986
Arvind Chopra1, Radhey S. Gupta1
1Department of Biochemistry, McMaster University, Hamilton, Canada

Tóm tắt

The nature of biochemical lesions in a number of independent single-step ouabainresistant (OuaR) mutants of Chinese hamster ovary cells has been examined. The cellular uptake of86 Rb in the mutant cells was found to be resistant to inhibition by ouabain and correlated with the degree of resistance of the mutants to the drug. However, the plasma membrane Na+, K+-ATPase from all five of the OuaR mutants examined showed similar sensitivity to inhibition by ouabain as seen for the enzyme from the parental sensitive cells. These results provide evidence that, contrary to the general assumption, OuaR mutants of Chinese hamster ovary cells, which are widely used for genetic and quantitative mutagenesis studies, are not directly affected in the Na+, K+-ATPase.

Tài liệu tham khảo

Akera, T., and Brody, T.M. (1978).Pharmacol. Rev. 29:187–200.

Noble, D. (1980).Cardiovasc. Res. 14:495–514.

Hansen, O. (1984).Pharmacol. Rev. 36:143–163.

Okita, G.T. (1967).Fed. Proc. 26:1125–1130.

Gupta, R.S., Chopra, A., and Stetsko, D.K. (1986).J. Cell. Physiol. 127:197–206.

Mankovitz, R., Buchwald, M., and Baker, R.M. (1984).Cell 3:221–226.

Rosenberg, H.M. (1975).J. Cell. Physiol. 85:135–142.

Baker, R.M. (1976). InBiogenesis and Turnover of Membrane Macromolecules (ed.) Cook, J.S. (Raven Press, New York), pp. 93–103.

Robbins, A.R., and Baker, R.M. (1977).Biochemistry 16:5163–5168.

Corsaro, C.M., and Migeon, N.R. (1978).Somat. Cell Genet. 4:531–540.

Choy, W.N., and Littlefield, J.W. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:1101–1105.

Gupta, R.S. (1981).Biochem. Pharmacol. 30:3039–3044.

Gupta, R.S., and Chopra, A. (1985).J. Biol. Chem. 260:6843–6850.

Chopra, A., and Gupta, R.S. (1986).J. Biol. Chem. 261:2034–2040.

Baker, R.M., Brunette, D.M., Mankovitz, R., Thompson, L.H., Whitmore, G.F., Siminovitch, L., and Till, J.E. (1974).Cell 1:9–21.

Mayhew, E. (1972).J. Cell. Physiol. 79:441–452.

Soderberg, K., Rossi, N., Lazdunski, M., and Louvard, D. (1983).J. Biol. Chem. 258:12300–12307.

Gupta, R.S., and Siminovitch, L. (1980).Mutat. Res. 69:113–126.

Levenson, R., Racaniello, V., Albritton, L., and Housman, D. (1984).Proc. Natl. Acad. Sci. U.S.A. 81:1489–1493.

English, L.H., Epstein, J., Cantley, L., Housman, D., and Levenson, R. (1985).J. Biol. Chem. 260:1114–1119.

Baker, R.M. (1979). InBanbury Report 2: Mammalian Cell Mutagenesis: The Maturation of Test Systems, (eds.), Hsie, A.W., O'Neill, J.P., and McElheny, V.K. (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York), pp. 237–247.

Gupta, R.S. (1984). InHandbook of Mutagenicity Test Procedures, (eds.) Kilbey, B.J., Legator, M., Nichols, W., and Ramel, C. (Elsevier Science Publishers, Amsterdam), pp. 291–319.

Gupta, R.S., and Siminovitch, L. (1978).Cell 14:1007–1013.

Gupta, R.S., Ho, T.K.W., Moffat, M.R.K., and Gupta, R. (1982).J. Biol. Chem. 257:1071–1078.

Gupta, R.S. (1984).Mol. Cell Biol. 4:1761–1768.

Thông tin
Thông tin xuất bản

Springer Science and Business Media LLC

Tập 12

367-373

Thông tin tác giả