Osteoblasts behavior on chemically treated commercially pure titanium surfaces

Journal of Biomedical Materials Research - Part A - Tập 102 Số 6 - Trang 1816-1822 - 2014
Diego Pedreira de Oliveira1, Annalisa Palmieri2, Francesco Carinci2, Claudemiro Bolfarini1
1Department of Materials Engineering, Federal University of São Carlos, São Carlos, Brazil
2Department of Maxillofacial Surgery, University of Ferrara, Ferrara, Italy

Tóm tắt

Abstract

Surface modifications of commercially pure titanium (Cp‐Ti), a material widely used to produce dental implants, can induce specific responses on osteoblastic cells after implantation. This work aims to investigate the influence of chemically modified surfaces of Cp‐Ti by acid etching or acid etching plus alkaline treatment on the gene expression of human osteoblastic (Hob) cells. Roughness and contact angle measurements were carried out to evaluate the surface properties of the samples. The surface morphology was investigated with scanning electron microscopy. Chemical composition was analyzed by energy dispersive X‐ray spectroscopy (EDS). The expression levels of some bone‐related genes (ALPL, COL1A1, COL3A1, SPP1, RUNX2, and SPARC) were analyzed using real time Reverse Transcription‐Polymerase Chain Reaction (real time RT‐PCR). The results showed that all the chemical modifications studied in this work influenced the surface morphology, wettability, roughness and induced an osteoconductive behavior. The samples that were acid etched and alkaline treated showed a more pronounced effect. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1816–1822, 2014.

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Tài liệu tham khảo

Ratner BD, 2004, Biomaterials Science: An Introduction to Materials in Medicine. 2, 851

10.1016/j.actbio.2004.11.009

Cannizzaro G, 2010, Immediate occlusal versus non‐occlusal loading of single zirconia implants. A multicentre pragmatic randomised clinical trial, Eur J Oral Implantol, 3, 111

10.1111/j.1600-0501.2010.01942.x

10.1111/j.1600-0501.2010.02113.x

10.1111/j.1708-8208.2009.00148.x

10.1111/j.1708-8208.2008.00096.x

10.1111/j.1600-0501.2010.02065.x

10.1016/j.dental.2006.06.025

10.1016/j.surfcoat.2006.04.052

10.1111/j.1600-0501.2007.01344.x

10.1039/C0NR00485E

10.1111/j.1600-0501.2005.01170.x

Marinucci L, 2006, Effect of titanium surface roughness on human osteoblast proliferation and gene expression in vitro, Int J Oral Maxillofac Implants, 21, 719

10.1007/s10856-006-8475-8

10.1007/s10856-008-3505-3

10.1016/j.actbio.2010.08.024

10.1002/jbm.b.31233

10.1016/j.biocel.2007.05.024

10.1002/jcb.240560310

10.1002/jcb.20958

10.1016/j.actbio.2009.10.011

10.1177/10454411000110030101

10.1073/pnas.140021997

Oliveira NTC, 2011, In vitro analysis with human bone marrow stem cells on Ti‐15Mo alloy for dental and orthopedic implants application, J Osseointegr, 1, 10

Bilezikian JP, 2002, Principles of Bone Biology, 1696

10.1002/jbm.a.34378

10.1006/meth.2001.1262

10.1038/sj.onc.1207676

10.1096/fasebj.8.2.8119487

10.1016/S0945-053X(00)00105-0

10.1369/jhc.2010.956144