Organization of the replication control region of plasmid ColIb-P9

Journal of Bacteriology - Tập 172 Số 4 - Trang 1983-1991 - 1990
Chihiro Hama1, Takenori Takizawa1, H Moriwaki1, Yukio URASAKI1, K Mizobuchi1
1Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.

Tóm tắt

We identified a 1,845-base-pair sequence that contains essential information for the autonomous replication and regulation of the 93-kilobase-pair IncI alpha group ColIb-P9 plasmid. Biochemical and genetic analyses revealed that this sequence specifies at least two structural genes, designated repZ and inc. The repZ gene encodes a protein with a molecular weight of 39,000, which probably functions as an initiator for the ColIb-P9 replicon. The inc gene that phenotypically governs the incompatibility encodes an RNA with a size of about 70 bases. This small RNA acts in trans to repress the expression of repZ, thereby functioning to maintain a constant copy number of the ColIb-P9 replicon in host cells.

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