Oral intake of Lactobacillus pentosus strain b240 accelerates salivary immunoglobulin A secretion in the elderly: A randomized, placebo-controlled, double-blind trial
Tóm tắt
Immunoglobulin A (IgA) secretion in saliva decreases with age and may be the cause of increased vulnerability of the elderly to respiratory infections. The effect of oral intake of lactic acid bacteria on salivary secretory IgA (SIgA) in the elderly has not been reported. The objective of this study was to demonstrate the acceleration of salivary SIgA secretion by oral intake of Lactobacillus pentosus strain b240 (b240) in the elderly. A total of 80 healthy elderly individuals were randomly allocated to either an intervention (i.e., b240) or a control (i.e., placebo) group. The elderly individuals in the b240 group were given a sterile water beverage (125 mL) containing heat-killed b240 (4 × 109 cells), while those in the placebo group were given only a sterile water beverage (125 mL); both groups received their respective beverages once daily for 12 weeks. Saliva was collected before initiation of the study and every 2 weeks thereafter. Saliva flow rate and SIgA concentration were determined, and the SIgA secretion rate was calculated. The mean salivary SIgA secretion rate in the b240 group steadily increased until week 4 (exhibiting a 20% elevation relative to that at week 0), and then remained stable until week 12. Changes in SIgA secretion rate over the intervention period were significantly greater in the b240 group than in the placebo group. The treatment groups exhibited no significant differences in adverse events. Oral intake of L. pentosus strain b240 for 12 weeks significantly accelerated salivary SIgA secretion, thereby indicating its potential utility in the improvement of mucosal immunity and resistance against infection in the elderly.
Tài liệu tham khảo
Mestecky J, Lamm ME, McGhee JR, Bienenstock J, Mayer L, Strober W: Mucosal Immunology. 2005, San Diego, CA: Academic Press, 3
Woof JM, Mestecky J: Mucosal immunoglobulins. Immunol Rev. 2005, 206: 64-82. 10.1111/j.0105-2896.2005.00290.x.
Holmgren J, Czerkinsky C: Mucosal immunity and vaccines. Nat Med. 2005, 11 (Suppl 4): S45-53. 10.1038/nm1213.
Brandtzaeg P: Mucosal immunity: induction, dissemination, and effector functions. Scand J Immunol. 2009, 70: 505-515. 10.1111/j.1365-3083.2009.02319.x.
Albers R, Antoine JM, Bourdet-Sicard R, Calder PC, Gleeson M, Lesourd B, Samartin S, Sanderson IR, Van Loo J, Vas Dias FW, Watzl B: Markers to measure immunomodulation in human nutrition intervention studies. Br J Nutr. 2005, 94: 452-481. 10.1079/BJN20051469.
Tomasi TB: The discovery of secretory IgA and the mucosal immune system. Immunol Today. 1992, 13: 416-418. 10.1016/0167-5699(92)90093-M.
Hanson LA, Björkander J, Oxelius VA: Selective IgA deficiency. Primary and secondary immunodeficiency disorders. Edited by: Chandra RK. 1983, Edinburgh: Churchill Livingstone, 62-64.
Gavazzi G, Krause KH: Ageing and infection. Lancet Infect Dis. 2002, 2: 659-666. 10.1016/S1473-3099(02)00437-1.
Phair J, Kauffman CA, Bjornson A, Adams L, Linnemann C: Failure to respond to influenza vaccine in the aged: correlation with B-cell number and function. J Lab Clin Med. 1978, 92: 822-828.
Cook JM, Gualde N, Hessel L, Mounier M, Michel JP, Denis F, Ratinaud MH: Alterations in the human immune response to hepatitis B vaccine among the elderly. Cell Immunol. 1987, 109: 89-96. 10.1016/0008-8749(87)90294-2.
Burns EA, Lum LG, L'Hommedieu G, Goodwin JS: Specific humoral immunity in the elderly: in vivo and in vitro response to vaccination. J Gerontol. 1993, 48: B231-236.
Kolibab K, Smithson SL, Shrine AK, Khuder S, Romero-Steiner S, Carlone GM, Westerink MA: Immune response to pneumococcal polysaccharides 4 and 14 in elderly and young adults. I. Antibody concentrations, avidity and functional activity. Immun Ageing. 2005, 2: 10-10.1186/1742-4933-2-10.
Aspinall R, Del Giudice G, Effros RB, Grubeck-Loebenstein B, Sambhara S: Challenges for vaccination in the elderly. Immun Ageing. 2007, 4: 9-10.1186/1742-4933-4-9.
Smith DJ, Joshipura K, Kent R, Taubman MA: Effect of age on immunoglobulin content and volume of human labial gland saliva. J Dent Res. 1992, 71: 1891-1894. 10.1177/00220345920710120701.
Challacombe SJ, Percival RS, Marsh PD: Age-related changes in immunoglobulin isotypes in whole and parotid saliva and serum in healthy individuals. Oral Microbiol Immunol. 1995, 10: 202-207. 10.1111/j.1399-302X.1995.tb00143.x.
Miletic ID, Schiffman SS, Miletic VD, Sattely-Miller EA: Salivary IgA secretion rate in young and elderly persons. Physiol Behav. 1996, 60: 243-248. 10.1016/0031-9384(95)02161-2.
Evans P, Der G, Ford G, Hucklebridge F, Hunt K, Lambert S: Social class, sex, and age differences in mucosal immunity in a large community sample. Brain Behav Immun. 2000, 14: 41-48. 10.1006/brbi.1999.0571.
Tanida T, Ueta E, Tobiume A, Hamada T, Rao F, Osaki T: Influence of aging on Candidal growth and adhesion regulatory agents in saliva. J Oral Pathol Med. 2001, 30: 328-335. 10.1034/j.1600-0714.2001.300602.x.
Ezendam J, van Loveren H: Probiotics: immunomodulation and evaluation of safety and efficacy. Nutr Rev. 2006, 64: 1-14. 10.1111/j.1753-4887.2006.tb00168.x.
Park JH, Um JI, Lee BJ, Goh JS, Park SY, Kim WS, Kim PH: Encapsulated Bifidobacterium bifidum potentiates intestinal IgA production. Cell Immunol. 2002, 219: 22-27. 10.1016/S0008-8749(02)00579-8.
Fukushima Y, Kawata Y, Hara H, Terada A, Mitsuoka T: Effect of a probiotic formula on intestinal immunoglobulin A production in healthy children. Int J Food Microbiol. 1998, 42: 39-44. 10.1016/S0168-1605(98)00056-7.
Tormo Carnicer R, Infante Pina D, Rosello Mayans E, Bartolome Comas R: Intake of fermented milk containing Lactobacillus casei DN-114 001 and its effect on gut flora. Ann Pedatr (Barc). 2006, 65: 448-453. 10.1157/13094251.
Okada S, Daengsubha W, Uchimura T, Ohara N, Kozaki M: Flora of lactic acid bacteria in Miang produced in northern Thailand. J Gen Appl Microbiol. 1986, 32: 57-65. 10.2323/jgam.32.57.
Bringel F, Castioni A, Olukoya DK, Felis GE, Torriani S, Dellaglio F: Lactobacillus plantarum subsp. argentoratensis subsp. nov., isolated from vegetable matrices. Int J Syst Evol Microbiol. 2005, 55: 1629-1634. 10.1099/ijs.0.63333-0.
Yamahira S, Toba M, Kishi K, Okamatsu H: Stimulation of mucosal immune system by lactic acid bacteria originating in tea. Jpn J Lactic Acid Bact. 2006, 17: 57-60.
Kishi K, Kotani Y, Yamahira S, Toba M, Okamatsu H, Mihara S, Tsuruta Y, Takehara M, Akahoshi A, Minami H: Lactobacillus plantarum ONRIC b0240 enhanced salivary IgA in healthy adult volunteers. Jpn J Lactic Acid Bact. 2006, 17: 132-137.
Petrunov B, Marinova S, Markova R, Nenkov P, Nikolaeva S, Nikolova M, Taskov H, Cvetanov J: Cellular and humoral systemic and mucosal immune responses stimulated in volunteers by an oral polybacterial immunomodulator "Dentavax". Int Immunopharmacol. 2006, 6: 1181-1193. 10.1016/j.intimp.2006.02.012.
Bishop NC, Gleeson M: Acute and chronic effects of exercise on markers of mucosal immunity. Front Biosci. 2009, 14: 4444-4456. 10.2741/3540.
Fahlman MM, Engels HJ: Mucosal IgA and URTI in American college football players: a year longitudinal study. Med Sci Sports Exerc. 2005, 37: 374-380. 10.1249/01.MSS.0000155432.67020.88.
Neville V, Gleeson M, Folland JP: Salivary IgA as a risk factor for upper respiratory infections in elite professional athletes. Med Sci Sports Exerc. 2008, 40: 1228-1236. 10.1249/MSS.0b013e31816be9c3.
Salminen S, Ouwehand A, Benno Y, Lee YK: Probiotics: how should they be defined?. Trends Food Sci Tech. 1999, 10: 107-110. 10.1016/S0924-2244(99)00027-8.
Kataria J, Li N, Wynn JL, Neu J: Probiotic microbes: do they need to be alive to be beneficial?. Nutr Rev. 2009, 67: 546-550. 10.1111/j.1753-4887.2009.00226.x.
Park SJ, Tokura H: Bright light exposure during the daytime affects circadian rhythms of urinary melatonin and salivary immunoglobulin A. Chronobiol Int. 1999, 16: 359-371. 10.3109/07420529909116864.
Jemmott JB, Borysenko JZ, Borysenko M, McClelland DC, Chapman R, Meyer D, Benson H: Academic stress, power motivation, and decrease in secretion rate of salivary secretory immunoglobulin A. Lancet. 1983, 1: 1400-1402. 10.1016/S0140-6736(83)92354-1.
Mackinnon LT, Hooper S: Mucosal (secretory) immune system responses to exercise of varying intensity and during overtraining. Int J Sports Med. 1994, 15 (Suppl 3): 179-183. 10.1055/s-2007-1021134.
McMurray DN, Rey H, Casazza LJ, Watson RR: Effect of moderate malnutrition on concentrations of immunoglobulins and enzymes in tears and saliva of young Colombian children. Am J Clin Nutr. 1977, 30: 1944-1948.
Michishige F, Kanno K, Yoshinaga S, Hinode D, Takehisa Y, Yasuoka S: Effect of saliva collection method on the concentration of protein components in saliva. J Med Invest. 2006, 53: 140-146. 10.2152/jmi.53.140.
Navazesh M: Methods for collecting saliva. Ann N Y Acad Sci. 1993, 694: 72-77. 10.1111/j.1749-6632.1993.tb18343.x.
Aufricht C, Tenner W, Salzer HR, Khoss AE, Wurst E, Herkner K: Salivary IgA concentration is influenced by the saliva collection method. Eur J Clin Chem Clin Biochem. 1992, 30: 81-83.
Shirtcliff EA, Granger DA, Schwartz E, Curran MJ: Use of salivary biomarkers in biobehavioral research: cotton-based sample collection methods can interfere with salivary immunoassay results. Psychoneuroendocrinology. 2001, 26: 165-173. 10.1016/S0306-4530(00)00042-1.
Takaoka W, Mori T, Kawata T: Studies on acid-base balance of saliva. IV. Studies on the seasonal variations of secretory rate and acid-base balance of saliva. J Physiol Soc Jpn. 1954, 16: 1-11.
Shannon IL: Climatological effects on human parotid gland function. Arch Oral Biol. 1966, 11: 451-453. 10.1016/0003-9969(66)90109-9.
Koyano W, Shibata H, Nakazato K, Haga H, Suyama Y: Measurement of competence: reliability and validity of the TMIG Index of Competence. Arch Gerontol Geriatr. 1991, 13: 103-116. 10.1016/0167-4943(91)90053-S.
Kinugasa T, Nagasaki H: Reliability and validity of the Motor Fitness Scale for older adults in the community. Aging. 1998, 10: 295-302.
Yokoyama K, Araki S, Kawakami N, Takeshita T: Production of the Japanese edition of Profile of Mood States (POMS): Assessment of reliability and validity. Jpn J Public Health. 1990, 37: 913-918.
Sasaki S, Yanagibori R, Amano K: Self-administered diet history questionnaire development for health education: a relative validation of the test-version by comparison with 3-day diet record in women. J Epidemiol. 1998, 8: 203-215.
Sasaki S, Ushio F, Amano K, Morihara M, Todoroki O, Uehara Y, Toyooka E: Serum biomarker-based validation of a self-administered diet history questionnaire for Japanese subjects. J Nutr Sci Vitaminol. 2000, 46: 285-296.
Naito Y, Harada A, Inoue S, Kitabatake Y, Arao T, Ohashi Y: Report of the physical activity research of the Japan Arteriosclerosis Longitudinal Study. Res Exerc Epidemiol. 2003, 5: 1-7.