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Tối ưu hóa một số điều kiện của phản ứng plastein xúc tác bởi Neutrase nhằm trung gian hoạt tính ức chế ACE in vitro của thủy phân casein được chuẩn bị bởi Neutrase
Tóm tắt
Thủy phân casein đã được thực hiện bằng cách thủy phân casein với Neutrase và sau đó được sửa đổi bằng phản ứng plastein xúc tác bởi Neutrase. Thủy phân đã chuẩn bị có tỷ lệ thủy phân là 13,0% và thể hiện hoạt tính ức chế ACE in vitro với giá trị IC50 là 40,4 μg⋅mL−1. Với lượng nhóm amin tự do giảm của thủy phân đã sửa đổi như là phản ứng, một số điều kiện của phản ứng plastein bao gồm nồng độ chất nền, tỷ lệ enzyme trên chất nền, nhiệt độ phản ứng và thời gian đã được nghiên cứu bằng các thí nghiệm đơn yếu tố và phương pháp bề mặt phản hồi, và cuối cùng được tối ưu hóa lần lượt là 62% (w/w), 3,0 kU⋅g−1 peptide, 30 °C và 6,3 h. Số lượng nhóm amin tự do giảm tối đa của thủy phân đã sửa đổi chuẩn bị dưới các điều kiện tối ưu hóa này là 210,0 μmol⋅g−1 peptide, trong khi giá trị IC50 tương ứng đã giảm xuống còn 14,7 μg⋅mL−1. Kết quả hiện tại cho thấy rằng phản ứng plastein xúc tác bởi Neutrase có khả năng tăng cường hoạt tính ức chế ACE in vitro của thủy phân casein, và cũng nhấn mạnh tầm quan trọng của một nghiên cứu trong tương lai nhằm điều tra thành phần peptide của thủy phân đã sửa đổi và vai trò của protease đã sử dụng trong phản ứng plastein.
Từ khóa
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