Optimization of chondrogenic differentiation of human adipose tissue-derived stem cells on poly(L-lactide-co-ɛ-caprolactone) scaffolds

Macromolecular Research - Tập 20 Số 7 - Trang 709-714 - 2012
Jung, Youngmee1, Kim, Sang-Hee1, Kim, Sang-Heon1, Kim, Young Ha2, Rhie, Jong Won3, Kim, Soo Hyun1
1Biomaterials Research Center, Korea Institute of Science and Technology, Seoul, Korea
2Department of Materials Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, Korea
3Department of Plastic Surgery, Catholic University College of Medicine, Seoul, Korea

Tóm tắt

Mechanical stimuli should enhance cartilaginous tissue formation in elastic mechano-active poly(L-lactide-co-ɛ-caprolactone) (PLCL) scaffolds similar to what is observed in the body. The conditions for stem cell differentiation are also crucial for cartilage tissue engineering. Here, we investigate cartilaginous tissue formation of human adipose-tissue derived stem cells (hASCs)/hybrid scaffold constructs with fibrin gel and PLCL scaffolds. For this, hASCs were mixed with 1% or 5% fibrin gel and were subsequently seeded onto the PLCL scaffolds. Next, chondrogenesis was induced in vitro in the constructs with chondrogenic medium for 14 or 21 days to determine the optimal concentration of fibrin gel and pre-conditioning period. After pre-conditioning, the constructs were subcutaneously implanted in nude mice for up to 8 weeks. The constructs grown in 5% fibrin gel and induced for 21 days or 1% fibrin gel and induced for 14 days showed higher depositions of cartilage-specific extracellular matrix (ECM) components compare to the constructs in 5% fibrin gels that were induced for 14 days or 1% fibrin gels induced for 21 days. These results indicated that the constructs maintained in dense hydrogels need longer pre-conditioning periods for chondrogenesis. Consequently, the hybrid constructs in which chondrogenesis is appropriately induced in vitro could be used to engineer cartilage.

Tài liệu tham khảo

citation_journal_title=J. Biomed. Mater. Res. A; citation_author=X. B. Jin, Y. S. Sun, K. Zhang, J. Wang, T. P. Shi, X. D. Ju, S. Q. Lou; citation_volume=86; citation_publication_date=2008; citation_pages=1077; citation_id=CR1 citation_journal_title=Adv. Drug Deliv. Rev.; citation_author=C. Chung, J. A. Burdick; citation_volume=60; citation_publication_date=2008; citation_pages=243; citation_doi=10.1016/j.addr.2007.08.027; citation_id=CR2 citation_journal_title=Macromol. Res.; citation_author=K. Y. Lee; citation_volume=13; citation_publication_date=2005; citation_pages=277; citation_doi=10.1007/BF03218454; citation_id=CR3 citation_journal_title=Curr. Opin. Rheumatol.; citation_author=C. K. Kuo, W. J. Li, R. L. Mauck, R. S. Tuan; citation_volume=18; citation_publication_date=2006; citation_pages=64; citation_doi=10.1097/01.bor.0000198005.88568.df; citation_id=CR4 citation_journal_title=Tissue Eng.; citation_author=J. L. Dragoo, G. Carlson, F. McCormick, H. Khan-Farooqi, M. Zhu, P. A. Zuk, P. Benhaim; citation_volume=13; citation_publication_date=2007; citation_pages=1615; citation_doi=10.1089/ten.2006.0249; citation_id=CR5 citation_journal_title=Biochem. Biophys. Res. Commun.; citation_author=G. R. Erickson, J. M. Gimble, D. M. Franklin, H. E. Rice, H. Awad, F. Guilak; citation_volume=290; citation_publication_date=2002; citation_pages=763; citation_id=CR6 citation_journal_title=N. Engl. J. Med.; citation_author=Y. F. Liu, W. M. Chen, Y. F. Lin, R. C. Yang, M. W. Lin, L. H. Li, Y. H. Chang, Y. S. Jou, P. Y. Lin, J. S. Su, S. F. Huang, K. J. Hsiao, C. S. Fann, H. W. Hwang, Y. T. Chen, S. F. Tsai; citation_volume=352; citation_publication_date=2005; citation_pages=2294; citation_doi=10.1056/NEJMoa042480; citation_id=CR7 citation_journal_title=Curr. Opin. Biotechnol.; citation_author=J. Raghunath, H. J. Salacinski, K. M. Sales, P. E. Butler, A. M. Seifalian; citation_volume=16; citation_publication_date=2005; citation_pages=503; citation_doi=10.1016/j.copbio.2005.08.004; citation_id=CR8 citation_journal_title=Artif. Organs; citation_author=C. Z. Jin, S. R. Park, B. H. Choi, K. Park, B. H. Min; citation_volume=31; citation_publication_date=2007; citation_pages=183; citation_doi=10.1111/j.1525-1594.2007.00363.x; citation_id=CR9 citation_journal_title=Adv. Drug Deliv. Rev.; citation_author=D. Nesic, R. Whiteside, M. Brittberg, D. Wendt, I. Martin, P. Mainil-Varlet; citation_volume=58; citation_publication_date=2006; citation_pages=300; citation_doi=10.1016/j.addr.2006.01.012; citation_id=CR10 citation_journal_title=Biomaterials; citation_author=H. Betre, S. R. Ong, F. Guilak, A. Chilkoti, B. Fermor, L. A. Setton; citation_volume=27; citation_publication_date=2006; citation_pages=91; citation_doi=10.1016/j.biomaterials.2005.05.071; citation_id=CR11 citation_journal_title=Biomaterials; citation_author=J. L. Drury, D. J. Mooney; citation_volume=24; citation_publication_date=2003; citation_pages=4337; citation_doi=10.1016/S0142-9612(03)00340-5; citation_id=CR12 citation_journal_title=Acta Biomater.; citation_author=K. L. Spiller, S. J. Laurencin, D. Charlton, S. A. Maher, A. M. Lowman; citation_volume=4; citation_publication_date=2008; citation_pages=17; citation_doi=10.1016/j.actbio.2007.09.001; citation_id=CR13 citation_journal_title=J. Biomater. Sci. Polym. Ed.; citation_author=Y. Jung, S. H. Kim, Y. H. Kim, J. Xie, T. Matsuda, B. G. Min; citation_volume=19; citation_publication_date=2008; citation_pages=61; citation_doi=10.1163/156856208783227712; citation_id=CR14 citation_journal_title=J. Biomater. Sci. Polym. Ed.; citation_author=Y. Jung, S. H. Kim, H. J. You, Y. H. Kim, B. G. Min; citation_volume=19; citation_publication_date=2008; citation_pages=1073; citation_doi=10.1163/156856208784909336; citation_id=CR15 citation_journal_title=Biomaterials; citation_author=Y. Jung, M. S. Park, J. W. Lee, Y. H. Kim, S. H. Kim; citation_volume=29; citation_publication_date=2008; citation_pages=4630; citation_doi=10.1016/j.biomaterials.2008.08.031; citation_id=CR16 citation_journal_title=J. Biomed. Mater. Res. A; citation_author=S. Terada, H. Yoshimoto, J. R. Fuchs, M. Sato, I. Pomerantseva, M. K. Selig, D. Hannouche, J. P. Vacanti; citation_volume=75; citation_publication_date=2005; citation_pages=907; citation_id=CR17 citation_journal_title=Acta Biomater.; citation_author=Y. Hong, H. Song, Y. Gong, Z. Mao, C. Gao, J. Shen; citation_volume=3; citation_publication_date=2007; citation_pages=23; citation_doi=10.1016/j.actbio.2006.06.007; citation_id=CR18 citation_journal_title=Tissue Eng.; citation_author=C. R. Lee, S. Grad, K. Gorna, S. Gogolewski, A. Goessl, M. Alini; citation_volume=11; citation_publication_date=2005; citation_pages=1562; citation_doi=10.1089/ten.2005.11.1562; citation_id=CR19 citation_journal_title=Biomed. Mater.; citation_author=Y. Jung, S. H. Kim, Y. H. Kim; citation_volume=4; citation_publication_date=2009; citation_pages=055009; citation_doi=10.1088/1748-6041/4/5/055009; citation_id=CR20 citation_journal_title=Biomaterials; citation_author=Y. Jung, Y. I. Chung, S. H. Kim, G. Tae, Y. H. Kim, J. W. Rhie; citation_volume=30; citation_publication_date=2009; citation_pages=4657; citation_doi=10.1016/j.biomaterials.2009.05.034; citation_id=CR21 citation_journal_title=Biochim. Biophys. Acta; citation_author=R. W. Farndale, D. J. Buttle, A. J. Barrett; citation_volume=883; citation_publication_date=1986; citation_pages=173; citation_doi=10.1016/0304-4165(86)90306-5; citation_id=CR22 citation_journal_title=J. Biomater. Sci. Polym. Ed.; citation_author=Y. Jung, S. H. Kim, Y. H. Kim; citation_volume=21; citation_publication_date=2010; citation_pages=581; citation_doi=10.1163/156856209X430579; citation_id=CR23 citation_journal_title=J. Biomed. Mater. Res. A; citation_author=W. J. Li, K. G. Danielson, P. G. Alexander, R. S. Tuan; citation_volume=67; citation_publication_date=2003; citation_pages=1105; citation_doi=10.1002/jbm.a.10101; citation_id=CR24 citation_journal_title=Dev. Biol.; citation_author=M. Solursh, T. F. Linsenmayer, K. L. Jensen; citation_volume=94; citation_publication_date=1982; citation_pages=259; citation_doi=10.1016/0012-1606(82)90090-2; citation_id=CR25 citation_journal_title=Clin. Orthop. Relat. Res.; citation_author=D. R. Carter, G. S. Beaupre, M. Wong, R. L. Smith, T. P. Andriacchi, D. J. Schurman; citation_volume=427; citation_publication_date=2004; citation_pages=S69; citation_doi=10.1097/01.blo.0000144970.05107.7e; citation_id=CR26 citation_journal_title=Ann. Biomed. Eng.; citation_author=C. T. Hung, R. L. Mauck, C. C. Wang, E. G. Lima, G. A. Ateshian; citation_volume=32; citation_publication_date=2004; citation_pages=35; citation_doi=10.1023/B:ABME.0000007789.99565.42; citation_id=CR27 citation_journal_title=Adv. Drug Deliv. Rev.; citation_author=S. H. Lee, H. Shin; citation_volume=59; citation_publication_date=2007; citation_pages=339; citation_doi=10.1016/j.addr.2007.03.016; citation_id=CR28
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Macromolecular Research

Tập 20 Số 7

709-714

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