One-Step Immunochromatographic Dipstick Tests for Rapid Detection of Vibrio cholerae O1 and O139 in Stool Samples

American Society for Microbiology - Tập 10 Số 3 - Trang 476-478 - 2003
Farida Nato1,2,3, A Boutonnier1,2,3, Minoarisoa Rajerison1,2,3, Pierre Grosjean1,2,3, Sylvie Dartevelle1,2,3, Alain Guénolé1,2,3, N. A. Bhuiyan1,2,3, David A. Sack1,2,3, G. Balakrish Nair1,2,3, Joëlle Fournier1,2,3, Steven A. Williams1,2,3
1Institut Pasteur, Antananarivo, Madagascar
2Institut Pasteur Paris, France;
3Laboratory Sciences Division, International Centre for Diarrhoeal Diseases Research, Bangladesh (ICDDR,B), Dhaka, Bangladesh

Tóm tắt

ABSTRACT

We describe the development and evaluation of a rapid diagnostic test for Vibrio cholerae O1 and O139 based on lipopolysaccharide detection using gold particles. The specificity ranged between 84 and 100%. The sensitivity of the dipsticks ranged from 94.2 to 100% when evaluated with stool samples obtained in Madagascar and Bangladesh. The dipstick can provide a simple tool for epidemiological surveys.

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American Society for Microbiology

Tập 10 Số 3

476-478

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