On‐plate‐selective enrichment of glycopeptides using boronic acid‐modified gold nanoparticles for direct MALDI‐QIT‐TOF MS analysis

Proteomics - Tập 9 Số 22 - Trang 5046-5055 - 2009
Jia Tang1, Yingchao Liu2, Dawei Qi1, Guoping Yao1, Chunhui Deng1, Xiangmin Zhang1
1Department of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, P. R. China
2Department of Neurosurgery, Shandong Provincial Hospital of Shandong University, Jinan, P. R. China

Tóm tắt

AbstractIn this study, an on‐plate‐selective enrichment method is developed for fast and efficient glycopeptide investigation. Gold nanoparticles were first spotted and sintered on a stainless‐steel plate, then modified with 4‐mercaptophenylboronic acid to provide porous substrate with large specific surface and dual functions. These spots were used to selectively capture glycopeptides from peptide mixtures and the captured target peptides could be analyzed by MALDI‐MS simply by deposition of 2,5‐dihydroxybenzoic acid matrix. Horseradish peroxidase was employed as a standard glycoprotein to investigate the enrichment efficiency. In this way, the enrichment, washing and detection steps can all be fulfilled on a single MALDI target plate. The relatively small sample amount needed, low detection limit and rapid selective enrichment have made this on‐plate strategy promising for online enrichment of glycopeptides, which could be applied in high‐throughput proteome research.

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Proteomics

Tập 9 Số 22

5046-5055

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