Oligonucleotide-Modified Gold Nanoparticles for Intracellular Gene Regulation

American Association for the Advancement of Science (AAAS) - Tập 312 Số 5776 - Trang 1027-1030 - 2006
Nathaniel L. Rosi1, David A. Giljohann1, C. Shad Thaxton1, Abigail K. R. Lytton‐Jean1, Min Su Han1, Chad A. Mirkin1
1Department of Chemistry and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208–3113 USA

Tóm tắt

We describe the use of gold nanoparticle-oligonucleotide complexes as intracellular gene regulation agents for the control of protein expression in cells. These oligonucleotide-modified nanoparticles have affinity constants for complementary nucleic acids that are higher than their unmodified oligonucleotide counterparts, are less susceptible to degradation by nuclease activity, exhibit greater than 99% cellular uptake, can introduce oligonucleotides at a higher effective concentration than conventional transfection agents, and are nontoxic to the cells under the conditions studied. By chemically tailoring the density of DNA bound to the surface of gold nanoparticles, we demonstrated a tunable gene knockdown.

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C.A.M. gratefully acknowledges a NIH Director's Pioneer Award and a National Cancer Institute–Center of Cancer Nanotechnology Excellence grant for support of this research. The authors thank T. O'Halloran T. Meade L. Shea V. Band H. Godwin C. B. Gurumurthy R. I. Morimoto W. Russin The Biological Imaging Facility and the Keck Biophysics Facility for assistance.

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American Association for the Advancement of Science (AAAS)

Tập 312 Số 5776

1027-1030

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