Những quan sát về sự phân bố nội bào của ion amoni trong mô rễ ngô bằng cách sử dụng phương pháp phân tích phổ cộng hưởng từ hạt nhân 14N in vivo

Planta - Tập 183 - Trang 359-367 - 1991
R. B. Lee1, R. G. Ratcliffe2
1University of Bristol, Long Ashton Research Station, AFRC Institute of Arable Crops Research, Bristol, UK
2Department of Plant Sciences, University of Oxford, Oxford, UK

Tóm tắt

Chúng tôi chỉ ra rằng sự phụ thuộc pH của tỷ lệ trao đổi xúc tác bởi base của ion amoni cung cấp cơ sở để phân biệt giữa các bể amoni trong tế bào chất và vacuole trong mô thực vật. Trong môi trường sống, các phổ cộng hưởng từ hạt nhân 14N được ghi lại với và không có sự tách biệt proton 1H, và thông tin về sự phân bố tiểu bào của NH₄⁺ đã được thu được từ phân tích hình dạng đường của phổ được ghép nối 1H. Chúng tôi đã áp dụng phương pháp này cho mô rễ ngô (Zea mays L.) và phát hiện rằng: (i) nồng độ amoni trong tế bào chất là thấp, điều này phù hợp với hoạt tính lớn của glutamine synthetase có trong rễ; và (ii) sự ức chế glutamine synthetase bằng methionine sulphoximine đã làm tăng nồng độ amoni trong tế bào chất, dẫn đến sự xuất hiện của amoni trong nhựa mạch.

Từ khóa

#amoni #cytoplasmic #vacuolar #glutamine synthetase #maize #14N-nuclear magnetic resonance

Tài liệu tham khảo

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