Chuỗi nucleotide của gen enterotoxin C3 do tụ cầu vàng: So sánh chuỗi của cả ba loại enterotoxin C tụ cầu

Springer Science and Business Media LLC - Tập 220 - Trang 329-333 - 1990
Carolyn J. Hovde1, Sean P. Hackett1, Gregory A. Bohach1
1Department of Bacteriology and Biochemistry, University of Idaho, Moscow, USA

Tóm tắt

Gen cấu trúc entC3, mã hóa enterotoxin C3 do tụ cầu vàng tiết, đã được nhân bản từ hệ gen của Staphylococcus aureus FRI-913 và đã được giải trình tự. Chuỗi axit amin sơ khai của độc tố đã được suy diễn từ dữ liệu chuỗi nucleotide. entC3 chứa 801 base và mã hóa một protein tiền chất gồm 266 axit amin. Axit glutamic đã được xác định là đầu N của enterotoxin C3 trưởng thành. Do đó, 27 dư lượng đầu tiên của tiền chất độc tố bao gồm peptide tín hiệu, và độc tố trưởng thành chứa 239 axit amin với trọng lượng phân tử là 27 563 dalton. Enterotoxin C3 khác với enterotoxin C2 ở bốn axit amin và khác với enterotoxin C1 ở chín dư lượng. 167 dư lượng C-terminus của ba độc tố là giống nhau, ngoại trừ một sự thay thế axit amin bảo tồn trong enterotoxin C3. Mức độ liên quan miễn dịch giữa ba enterotoxin loại C tỷ lệ thuận với mức độ liên quan phân tử của chúng. Nghiên cứu này cũng cung cấp bằng chứng rằng đầu N của các enterotoxin loại C xác định các epitop kháng nguyên đặc hiệu theo kiểu con, trong khi các vùng C-terminus bảo tồn hơn xác định các đặc tính sinh học và các epitop kháng nguyên phản ứng chéo được chia sẻ với các độc tố gây sốt khác.

Từ khóa


Tài liệu tham khảo

Avena RM, Bergdoll MS (1967) Purification and some physicochemical properties of enterotoxin C, Staphylococcus aureus strain 361. Biochemistry 6:1474–1480 Barsumian EL, Cunningham CM, Schlievert PM, Watson DW (1978) Heterogeneity of group A streptococcal pyrogenic exotoxin type B. Infect Immun 20:512–518 Bergdoll MS, Schlievert PM (1984) Toxic shock syndrome toxin. Lancet 2:691 Bergdoll MS, Robbins RN, Weiss K, Borja CR, Huang IY, Chu FS (1973) The staphylococcal enterotoxins: similarities. Contrib Microbiol Immunol 1:390–396 Betley MJ, Mekalanos JJ (1988) Nucleotide sequence of the type A staphylococcal enterotoxin gene. J Bacteriol 170:34–41 Bohach GA, Schlievert PM (1987a) Expression of staphylococcal enterotoxin C1 in Escherichia coli. Infect Immun 55:428–432 Bohach GA, Schlievert PM (1987b) Nucleotide sequence of the staphylococcal enterotoxin C1 gene and relatedness to other pyrogenic toxins. Mol Gen Genet 209:15–20 Bohach GA, Schlievert PM (1989) Conservation of the biologically active portions of staphylococcal enterotoxins C1 and C2. Infect Immun 57:2249–2252 Bohach GA, Hovde CJ, Handley JP, Schlievert PM (1988) Cross-neutralization of Staphylococcal and streptococcal pyrogenic toxins by monoclonal and polyclonal antibodies. Infect Immun 56:400–404 Bohach GA, Handley JP, Schlievert PM (1989a) Biological and immunological properties of the carboxyl terminus of staphylococcal enterotoxin C1. Infect Immun 57:23–28 Bohach GA, Kreiswirth BN, Novick RP, Schlievert PM (1989b) Analysis of toxic shock syndrome isolates producing staphylococcal enterotoxins B and C1 with use of southern hybridization and immunological assays. Rev Infect Dis 11(1):75–81 Borja CR, Bergdoll MS (1967) Purification and partial characterization of enterotoxin C produced by Staphylococcus aureus strain 137. Biochemistry 6:1467–1473 Chang S, Cohen SN (1979) High efficiency transformation of Bacillus subtilis protoplasts by plasmid DNA. Mol Gen Genet 168:111–115 Couch JL, Soltis MT, Betley MJ (1988) Cloning and nucleotide sequence of the type E staphylococcal enterotoxin gene. J Bacteriol 170:2954–2960 deAzavedo JCS, Foster TJ, Hartigan PJ, Arbuthnott JP, O'Reilly M, Kreiswirth BN, Novick RP (1985) Expression of the cloned toxic shock syndrome toxin 1 gene (tst) in vivo with a rabbit uterine model. Infect Immun 50:304–309 Fast DJ, Schlievert PM, Nelson RD (1989) Toxic shock syndrome-associated staphylococcal and streptococcal pyrogenic toxins are potent inducers of tumor necrosis factor production. Infect Immun 57:291–294 Goshorn SC, Schlievert PM (1988) Nucleotide sequence of streptococcal pyrogenic exotoxin type C. Infect Immun 56:2518–2520 Hewick RM, Hunkapiller MW, Hood LE, Dreyer WJ (1981) A gas-liquid solid phase peptide and protein sequenator. J Biol Chem 256:7990–7997 Horinouchi S, Weisblum B (1982) Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibiotics. J Bacteriol 150:804–814 Johnson LP, L'Italien JJ, Schlievert PM (1986a) Streptococcal pyrogenic exotoxin type A (scarlet fever toxin) is related to Staphylococcus aureus enterotoxin B. Mol Gen Genet 203:354–356 Johnson LP, Tomai MA, Schlievert PM (1986b) Bacteriophage involvement in group A streptococcal pyrogenic exotoxin A production. J Bacteriol 166:623–627 Jones CJ, Khan SA (1986) Nucleotide sequence of the enterotoxin B gene from Staphylococcus aureus. J Bacteriol 166:29–33 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685 Messing J (1979) A multi-purpose cloning system based on the single-stranded DNA bacteriophage M13. Recombinant DNA technical bulletin, NIH publication no. 79-99, pp 43–48. National Institutes of Health, Bethesda, Md Ouchterlony O (1962) Diffusion-in-gel methods for immunological analysis. Prog Allergy 6:30–54 Poindexter NJ, Schlievert PM (1985) The biochemical and immunological properties of toxic shock syndrome toxin-1 (TSST-1) and association with TSS. J Toxicol Toxin Rev 4:1–39 Reiser RF, Robbins RN, Noleto AL, Khoe GP, Bergdoll MS (1984) Identification, purification, and some physicochemical properties of staphylococcal enterotoxin C3. Infect Immun 45:625–630 Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467 Schlievert PM (1980) Purification and characterization of staphylococcal pyrogenic exotoxin type B. Biochemistry 9:6204–6208 Schlievert PM, Shands KN, Dan BB, Schmid GP, Nishimura RD (1981) Identification and characterization of an exotoxin from Staphylococcus aureus associated with toxic shock syndrome. J Infect Dis 143:509–516 Soberon X, Covarrubias L, Bolivar F (1980) Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene 9:287–305 Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517 Thompson NE, Ketterhagen MJ, Bergdoll MS (1984) Monoclonal antibodies to staphylococcal enterotoxins B and C: cross-reactivity and localization of epitopes on tryptic fragments. Infect Immun 45:281–285 Vieira J, Messing J (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 16:259–268 Weeks CR, Ferretti JJ (1986) Nucleotide sequence of the type A streptococcal exotoxin (erythrogenic toxin) gene from Staphylococcus pyogenes. Infect Immun 52:144–150