Nucleic acid detection with CRISPR-Cas13a/C2c2

American Association for the Advancement of Science (AAAS) - Tập 356 Số 6336 - Trang 438-442 - 2017
Jonathan S. Gootenberg1,2,3,4,5, Omar O. Abudayyeh1,2,3,6,5, Jeong Wook Lee7, Patrick Essletzbichler1,2,3,5, Aaron J. Dy1,2,8, Julia Joung1,2,3,5, Vanessa K. Verdine1,2,3,5, Nina M. Donghia7, Nichole M. Daringer8, Catherine A. Freije1,9, Cameron Myhrvold1,9, Roby P. Bhattacharyya1, Jonathan Livny1, Aviv Regev1,10, Eugene V. Koonin11, Deborah T. Hung1, Pardis C. Sabeti1,9,12,13, James J. Collins1,2,6,8,7, Feng Zhang1,2,3,5
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
2Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 USA
3Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
4Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
5McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA.
6Department of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
7Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA
8Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
9Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA
10Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139 USA
11National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
12Department of Immunology and Infectious Disease, Harvard School of Public Health, Boston, MA 02115 USA
13Howard Hughes Medical Institute, Chevy Chase, MD 20815 USA

Tóm tắt

Sensitive and specific CRISPR diagnostics Methods are needed that can easily detect nucleic acids that signal the presence of pathogens, even at very low levels. Gootenberg et al. combined the allele-specific sensing ability of CRISPR-Cas13a with recombinase polymerase amplification methods to detect specific RNA and DNA sequences. The method successfully detected attomolar levels of Zika virus, as well as the presence of pathogenic bacteria. It could also be used to perform human genotyping from cell-free DNA. Science , this issue p. 438

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