Baixin Shen1, W Wang2, Ling Ding1, Yunpeng Sao1, Yi Huang1, Zhaofeng Shen1, Yisha Zhuo1, Zhongqing Wei1, W Zhang2
1Department of Urology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of China
2Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of China
Tóm tắt
Aim: This study aimed to determine whether nuclear factor erythroid 2-related factor 2 antagonized the oxidative stress induced by di- N-butylphthalate (DBP) in testicular Leydig cells. Methods: Mouse TM3 testicular Leydig cells were treated with Nrf2 knockdown (KD) or overexpression in the presence and absence of DBP. Oxidative profiles were examined. Nrf2 target antioxidant genes were studied, and the effects of Nrf2 inducer sulphoraphane (SFN) were tested. Results: DBP induced intracellular oxidative stress to a similar extent with Nrf2 KD. Expression and protein levels of Nrf2 were increased together with its target genes, namely heme oxygenase 1, nicotinamide adenine dinucleotide phosphate quinone oxidoreductase 1 and peroxiredoxin 6, following DBP stimulation. Use of SFN not only restored the intracellular oxidative toxicity but also cell proliferation and testosterone secretion in response to DBP. Conclusion: Increased Nrf2 activity, for example, by SFN can effectively antagonize the oxidative stress in testicular Leydig cells caused by DBP.
