Nuclear basic protein changes during spermiogenesis in the longnose skate and the spiny dogfish

Wiley - Tập 212 Số 3 - Trang 423-433 - 1980
Niels C. Bols1, S.A. Boliska1, J. B. Rainville1, Harold E. Kasinsky2
1Department of Biology, University of Waterloo, Waterloo, Ont., Canada, N2L 3G1
2Department of Zoology, University of British Columbia, Vancouver, B.C., Canada V6T 1W5

Tóm tắt

AbstractNuclear shape and the organization of nuclei within the seminiferous follicles have been used to divide spermiogenesis in the longnose skate, Raja rhina, into eight stages and in the spiny dogfish, Squalus acanthias, into seven stages. Cytochemical techniques for basic proteins reveal that as spermatid nuclei begin to elongate they have the somatic histone complement, and as they complete elongation they contain a very arginine‐rich, TCA‐extractable complement, or the salmon sperm histone type. After the nuclei have developed their ultimate corkscrew shape the final transition takes place to a very arginine‐rich, TCA‐stable complement, or the mammalian sperm histone type. These nuclei were not rich in sulfhydryl groups, but they were extractable with sodium thioglycolate. In addition, a number of variables affecting TCA extraction and subsequent alkaline fast green staining are described.

Từ khóa


Tài liệu tham khảo

10.1083/jcb.64.2.389

10.1083/jcb.2.2.109

Alfert M., 1958, Variations in cytochemical properties of cell nuclei, Exp. Cell Res., Suppl., 6, 227, 10.1016/S0014-4827(21)00285-8

10.1073/pnas.39.10.991

10.1007/978-3-642-46294-8

Barka T., 1965, Histochemistry, 39

10.1126/science.116.3013.323

10.1002/jez.1401880203

Bennett H. S., 1958, General Cytochemical Methods, 317

Bloch D. P., 1969, A catalog of sperm histones, Genetics (Suppl.), 61, 93

10.1007/978-1-4615-7148-3_5

10.1083/jcb.1.1.17

10.1083/jcb.7.3.515

10.1111/j.1432-0436.1977.tb01494.x

10.1139/z72-026

10.1139/z73-029

10.1139/z74-054

10.1002/jez.1401980112

Cecil R., 1963, The Proteins, 379

10.1016/0005-2795(72)90174-2

Das C. C., 1964, Histoneprotein transition in Drosophila melanogaster–I. Changes during spermatogenesis, Exp. Cell Res., 81, 333

10.1177/9.5.477

Dixon G. H., 1968, Progress in Nucleic Acid Research and Molecular Biology, 1

10.2307/3226095

Gormori G., 1956, Histochemical methods for protein‐bound sulphydryl and disulphide groups, Q. J. Micr. Sci., 97, 1

Gusse M., 1978, Ultrastructural and chemical study of chromatin during spermiogenesis of fish Scyliorhinus caniculus, Cytobiology, 16, 421

10.3109/10520297109067822

10.1002/jez.1402030111

10.1083/jcb.31.1.159

10.1016/0003-9861(67)90022-7

10.1002/jcp.1040780104

10.1007/BF01012056

10.1007/BF00336875

10.1016/S0022-5320(71)80090-4

10.1016/S0022-5320(71)80091-6

Subirana J. A., 1975, The Biology of the Male Gamete, 239

10.1177/5.4.403

10.1083/jcb.31.2.257

Vaughn J. C., 1972, Isolation and characterization of chromatin and DNA from sperm of spider crab, Libinia emarginata, J. Cell Sci., 11, 131, 10.1242/jcs.11.1.131

Thông tin
Thông tin xuất bản

Wiley

Tập 212 Số 3

423-433

Thông tin tác giả