Novel cell death program leads to neutrophil extracellular traps

Journal of Cell Biology - Tập 176 Số 2 - Trang 231-241 - 2007
Tobias A. Fuchs1,2, Ulrike Abu Abed1,3, Christian Goosmann1,3, Robert Hurwitz4, Ilka Schulze5, V. Wahn5, Yvette Weinrauch2, Volker Brinkmann3, Arturo Zychlinsky1
11Department for Cellular Microbiology
25Department of Microbiology, New York University School of Medicine, New York, NY 10016
32Microscopy Core Facility,
43Protein Purification Core Facility, Max-Planck Institute for Infection Biology, 10117 Berlin, Germany
54Department for Paediatric Pneumology and Immunology, Charité Universitätsmedizin Berlin, 13353 Berlin, Germany

Tóm tắt

Neutrophil extracellular traps (NETs) are extracellular structures composed of chromatin and granule proteins that bind and kill microorganisms. We show that upon stimulation, the nuclei of neutrophils lose their shape, and the eu- and heterochromatin homogenize. Later, the nuclear envelope and the granule membranes disintegrate, allowing the mixing of NET components. Finally, the NETs are released as the cell membrane breaks. This cell death process is distinct from apoptosis and necrosis and depends on the generation of reactive oxygen species (ROS) by NADPH oxidase. Patients with chronic granulomatous disease carry mutations in NADPH oxidase and cannot activate this cell-death pathway or make NETs. This novel ROS-dependent death allows neutrophils to fulfill their antimicrobial function, even beyond their lifespan.

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Journal of Cell Biology

Tập 176 Số 2

231-241

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