Nivalenol-Type Populations of Fusarium graminearum and F. asiaticum Are Prevalent on Wheat in Southern Louisiana

Phytopathology - Tập 101 Số 1 - Trang 124-134 - 2011
Liane Rosewich Gale1, Stephen A. Harrison2, Todd J. Ward3, Kerry O’Donnell3, E. A. Milus4, S. Gale3, Harold Kistler5
1Department of Plant Pathology, University of Minnesota, St. Paul, USA
2Louisiana State University
3United States Department of Agriculture
4University of Arkansas
5Plant Pathology

Tóm tắt

U.S. populations of the Fusarium graminearum clade cause head blight on wheat and barley and usually contaminate grain with the trichothecene mycotoxin deoxynivalenol (DON). Recently, however, individual nivalenol (NIV)-type isolates from the United States were described that belonged to either the newly described species F. gerlachii or the genetically distinct Gulf Coast population of F. graminearum sensu stricto (s.s.). Here, we describe the discovery of NIV-type F. graminearum s.s. populations that were found in high proportion (79%) among isolates from small-grain-growing regions of Louisiana. We genotyped 237 isolates from Louisiana with newly developed polymerase chain reaction (PCR) restriction fragment length polymorphism markers and multiplex PCR primers that distinguish among the three trichothecene types: the two DON types (15ADON and 3ADON) and NIV. These isolates were compared with 297 isolates from 11 other U.S. states, predominantly from the Midwest. Using Bayesian-model-based clustering, we discovered a southern Louisiana population of F. graminearum s.s. that was genetically distinct from the previously recognized pathogen population in the Midwest (MW15ADON population). Population membership was correlated with trichothecene type. Most isolates from the southern Louisiana population were of the NIV type, while the majority of the isolates from the Midwest were of the 15ADON type. A smaller proportion of isolates from Louisiana belonged to the previously described Gulf Coast population that was mostly of the 3ADON type. The NIV type was also identified in collections from Arkansas (12%), North Carolina (40%), and Missouri (2%), with the collections from Arkansas and North Carolina being small and unrepresentative. F. asiaticum was detected from the two southern Louisiana parishes Acadia and Alexandria. All identified 41 F. asiaticum isolates were of the NIV type. Greenhouse tests indicated that U.S. NIV types accumulated four times less trichothecene toxin than DON types on inoculated wheat. This is the first report of NIV-type populations of F. graminearum s. s. and F. asiaticum in the United States.

Từ khóa


Tài liệu tham khảo

10.1094/PD-78-0760

10.1146/annurev.es.16.110185.000553

Bowden, R. L., Leslie, J. F., Lee, J., and Lee, Y.W. 2006. Cross fertility of lineages in Fusarium graminearum (Gibberella zeae). Pages 54-60 in: The Global Fusarium Initiative for International Collaboration: A Strategic Planning Workshop. T. Ban, J. M. Lewis, and E. E. Phipps, eds. CIMMYT, El Batán, Mexico.

10.1094/Phyto-77-1640

Cowger, C., and Sutton, A. L. 2005. The southeastern U.S. Fusarium head blight epidemic of 2003. Plant Health Progress. Online publication 10.1094/PHP-2005-1026-01-RS.

10.1126/science.1143708

Desjardins, A. E. 2006. Fusarium Mycotoxins Chemistry, Genetics and Biology. American Phytopathological Society Press, St. Paul, MN.

10.1021/jf8003702

10.1016/j.fct.2003.11.006

10.1111/j.1365-294X.2005.02553.x

10.1177/117693430500100003

Fornelli, F., Minervini, F., and Mulè, G. Cytotoxicity induced by nivalenol, deoxynivalenol, and fumonisin B1 in the SF-9 insect line. In Vitro Cell. Dev. Biol. Anim. 40:166-171.

10.1534/genetics.105.044842

10.1094/PHYTO.2002.92.12.1315

10.1094/PHYTO-97-11-1434

10.1094/PHYTO-95-1397

Hsia C. C., 2004, Oncol. Rep., 12, 449

10.1094/PDIS-93-2-0170

10.1007/s10327-009-0153-5

10.1094/PHYTO.1997.87.9.915

10.1128/AEM.02287-08

10.1016/j.toxicon.2008.04.162

10.1111/j.1364-3703.2006.00351.x

10.1080/02652030600699072

10.1146/annurev.phyto.40.120501.101443

10.1094/PDIS.1997.81.12.1340

10.1111/j.1439-0434.2007.01394.x

10.1016/S0887-2333(03)00130-9

Mirocha C. J., 1989, Appl. Environ. Microbiol., 55, 1315, 10.1128/aem.55.5.1315-1316.1989

10.1071/AP06046

10.1111/j.1467-9353.2004.00183.x

10.1073/pnas.130193297

10.1016/j.fgb.2008.09.002

10.1016/j.fgb.2004.03.003

10.1093/oxfordjournals.molbev.a026050

10.1016/j.ijfoodmicro.2009.11.009

10.1016/S0377-8401(98)00278-8

10.1093/genetics/155.2.945

Qualset, C. O., and Shands, H. L. 2005. Safeguarding the Future of U.S. Agriculture: The Need to Conserve Threatened Collections of Crop Diversity Worldwide. University of California, Division of Agriculture and Natural Resources, Genetic Resources Conservation Program, Davis.

10.1016/j.toxlet.2004.04.045

10.1016/j.fgb.2007.03.001

10.1111/j.1471-8286.2004.00703.x

10.1094/PHYTO-98-2-0159

10.1021/jf00083a019

10.1006/fgbi.2000.1228

10.1094/PDIS.2001.85.4.404

10.1073/pnas.142307199

10.1016/j.fgb.2007.10.003

10.1094/PHYTO.2000.90.1.17

10.1146/annurev-phyto-080508-081737

10.1094/PHYTO-98-6-0719

10.3852/08-217

10.1271/bbb.68.2076

10.1046/j.1365-294X.2004.02098.x

10.1046/j.1365-294X.2003.01773.x

10.1016/j.mycres.2007.06.008

Thông tin
Thông tin xuất bản

Phytopathology

Tập 101 Số 1

124-134

Thông tin tác giả