Nitric oxide synthase-containing neurons in rat parasympathetic, sympathetic and sensory ganglia: a comparative study

Springer Science and Business Media LLC - Tập 27 - Trang 819-831 - 1995
Per Alm1, Bengt Uvelius2, Jörgen Ekström3, Bo Holmqvist4, Bengt Larsson5, Karl-Erik Andersson5
1Department of Pathology, University of Lund, Lund University Hospital, Lund, Sweden
2Department of Urology, University of Lund, Lund University Hospital, Lund, Sweden
3Department of Pharmacology, University of Gothenburg, Sweden
4Department of Zoology, University of Lund, Lund University Hospital, Lund, Sweden
5Department of Clinical Pharmacology, University of Lund, Lund University Hospital, Lund, Sweden

Tóm tắt

In rats, the distribution of nerve structures staining for NADPH-diaphorase, and showing immunoreactivities for nitric oxide synthase (NOS), tyrosine hydroxylase and various neuropeptides was studied in sensory ganglia (dorsal root, nodose and trigeminal ganglia), in sympathetic ganglia (superior cervical, stellate, coeliac-superior and inferior mesenteric ganglia), parasympathetic ganglia (sphenopalatine, submandibular, sublingual and otic ganglia), and in the mixed parasympathetic/ sympathetic ganglia (major pelvic ganglia). The coincidence of neuronal cell bodies with strong NOS-immunoreactivity and strong NADPH diaphorase reactivity was almost total. The relative proportions of NOS-immunoreactive nerve cell bodies were largest in parasympathetic ganglia and major pelvic ganglia followed by sensory ganglia. In sympathetic ganglia no NOS-immunoreactive neuronal cell bodies could be detected. In parasympathetic and major pelvic ganglia, there was a very significant neuronal co-localization of immunoreactivities for NOS and vasoactive intestinal polypeptide (VIP). This was almost total in major pelvic ganglia, in which NOS-/VIP-immunoreactive nerve cell bodies were separate from sympathetic (tyrosine hydroxylase-/neuropeptide Y-immunoreactive), suggesting that NOS-/VIP-immuno-reactive neurons might also be parasympathetic.

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