New insights in the composition of extracellular vesicles from pancreatic cancer cells: implications for biomarkers and functions

Springer Science and Business Media LLC - Tập 12 - Trang 1-18 - 2014
Susanne Klein-Scory1, Mahnaz Moradian Tehrani2, Christina Eilert-Micus1, Kamila A Adamczyk1, Nathalie Wojtalewicz1, Martina Schnölzer2, Stephan A Hahn3, Wolff Schmiegel1,4, Irmgard Schwarte-Waldhoff1
1IMBL, Medical Clinic Knappschaftskrankenhaus Bochum GmbH, Ruhr-University Bochum, Bochum, Germany
2Functional Proteome Analysis, German Cancer Research Center (DKFZ), Heidelberg, Germany
3Molecular Gastrointestinal Oncology MGO, Ruhr-University Bochum, Bochum, Germany
4Medical Department, Medical Clinic Knappschaftskrankenhaus Bochum GmbH, Ruhr-University Bochum, Bochum, Germany

Tóm tắt

Pancreatic cancer development is associated with characteristic alterations like desmoplastic reaction and immune escape which are mediated by the cell-cell communication mechanism and by the microenvironment of the cells. The whole of released components are important determinants in these processes. Especially the extracellular vesicles released by pancreatic cancer cells play a role in cell communication and modulate cell growth and immune responses. Here, we present the proteomic description of affinity purified extracellular vesicles from pancreatic tumour cells, compared to the secretome, defined as the whole of the proteins released by pancreatic cancer cells. The proteomic data provide comprehensive catalogues of hundreds of proteins, and the comparison reveals a special proteomic composition of pancreatic cancer cell derived extracellular vesicles. The functional analysis of the protein composition displayed that membrane proteins, glycoproteins, small GTP binding proteins and a further, heterogeneous group of proteins are enriched in vesicles, whereas proteins derived from proteasomes and ribosomes, as well as metabolic enzymes, are not components of the vesicles. Furthermore proteins playing a role in carcinogenesis and modulators of the extracellular matrix (ECM) or cell-cell interactions are components of affinity purified extracellular vesicles. The data deepen the knowledge of extracellular vesicle composition by hundreds of proteins that have not been previously described as vesicle components released by pancreatic cancer cells. Extracellular vesicles derived from pancreatic cancer cells show common proteins shared with other vesicles as well as cell type specific proteins indicating biomarker candidates and suggesting functional roles in cancer cell stroma interactions.

Tài liệu tham khảo

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