Neuroanatomical specificity in the co‐localization of aromatase and estrogen receptors

Wiley - Tập 22 Số 2 - Trang 143-157 - 1991
Jacques Balthazart1, A. Foidart1, Chantal Surlemont1, Nobuhiro Harada2
1Laboratory of General and Comparative Biochemistry, University of Liège, B-4020 Liège, Belgium
2Molecular Genetics, School of Medicine, Fujita-Gakuen Health University, Toyoake, AICHI 470-11, Japan

Tóm tắt

AbstractThe relative distributions of aromatase and of estrogen receptors were studied in the brain of the Japanese quail by a double‐label immunocytochemical technique. Aromatase immunoreactive cells (ARO‐ir) were found in the medial preoptic nucleus, in the septal region, and in a large cell cluster extending from the dorso‐lateral aspect of the ventromedial nucleus of the hypothalamus to the tuber at the level of the nucleus inferioris hypothalami. Immunoreactive estrogen receptors (ER) were also found in each of these brain areas but their distribution was much broader and included larger parts of the preoptic, spetal, and tuberal regions. In the ventromedial and tuberal hypothalamus, the majority of the ARO‐ir cells (over 75%) also contained immunoreactive ER. By contrast, very few of the ARO‐ir cells were double‐labeled in the preoptic area and in the septum. More than 80% of the aromatase‐containing cells contained no ER in these regions. This suggests that the estrogens, which are formed centrally by aromatization of testosterone, might not exert their biological effects through binding with the classical nuclear ER. The fact that significant amounts of aromatase activity are found in synaptosomes purified by differential centrifugation and that aromatase immunoreactivity is observed at the electron microscope level in synaptic boutons suggests that aromatase might produce estrogens that act at the synaptic level as neurohomones or neuromodulators.

Từ khóa


Tài liệu tham khảo

10.1016/0031-9384(86)90429-4

10.1016/0006-8993(89)90088-7

Balthazart J., 1989, Advances in Comparative and Environmental Physiology, 105

10.1016/0006-8993(89)90899-8

10.1016/0361-9230(90)90246-V

10.1016/0031-9384(90)90198-D

10.1016/0376-6357(88)90048-4

Balthazart J., 1988, Induction by testosterone of the aromatase in the sexually dimorphic preoptic medial nucleus and activation of copulatory behavior in quail, Soc. Neurosci. Abstr., 14, 97

10.1016/0006-8993(89)90638-0

10.1016/0018-506X(90)90039-Z

10.1016/0006-8993(90)91428-J

10.1016/0031-9384(90)90045-6

Balthazart J., 1990, Hormones, Brain and Behavior in Vertebrates. 2. Behavioural Activaion in Males and Females—Social Interactions and Reproductive Endocrinology. Comp. Physiol. Vol 9, 45

10.1002/cne.903010210

Balthazart J., 1990, Immunocytochemical localization of aromatase and estrogen receptors in the brain, Soc. Neurosci. Abstr., 16, 1313

10.1111/j.1365-2826.1990.tb00464.x

Baylé J. D., 1974, Stereotaxic topography of the brain of the quail, J. Physiol. (Paris), 68, 219

10.1016/0006-8993(89)91219-5

10.1159/000125152

10.1016/0006-8993(87)90158-2

10.1016/0039-128X(87)90035-3

10.1016/0303-7207(82)90032-6

Celotti F., 1979, Endocrinology, 41

10.1016/0006-8993(79)90068-4

10.1016/0197-0186(86)90196-8

10.1210/jcem-59-6-1088

10.1073/pnas.85.19.7380

10.1016/0006-8993(87)91063-8

10.1210/endo-126-3-1403

10.1016/0022-4731(84)90188-2

10.1093/oxfordjournals.jbchem.a122213

10.1016/0006-8993(84)90383-4

10.1210/endo-118-6-2180

10.1210/edrv-4-4-311

10.1016/0006-8993(89)90012-7

10.1002/cne.902060309

Kuenzel W. J., 1988, A Stereotaxic Atlas of the Brain of the Chick (Gallus domesticus), 166

10.1002/j.1460-2075.1986.tb04489.x

MacLusky N. J., 1984, Metabolism of Hormonal Steroids in the Neuroendocrine Structures, 103

10.1210/endo-114-6-2002

McEwen B. S., 1984, Metabolism of Hormonal Steroids in the Neuroendocrine Structures, 117

10.1016/S0002-9378(78)80010-6

10.1210/endo-90-1-295

Naftolin F., 1975, The formation of estrogens by central neuroendocrine tissues, Rec. Prog. Horm. Res., 31, 295

10.1095/biolreprod42.1.21

Naftolin F. Leranth C. andBalthazart J.(1990b). Ultrastructural localization of aromatase immunoreactivity in hypothalamic neurons.The Endocrine Soc. Abstr.669(Abstract.).

10.1095/biolreprod20.4.905

10.1016/0006-8993(87)91496-X

Halvorsen S. W., 1987, Steroid Hormone Receptors. Their Intracellular Localization, 251

10.1210/endo-109-1-273

10.1095/biolreprod30.2.388

Reger R. L., 1989, Abstr. Conf. Reprod. Behav, 62

10.1210/endo-114-6-2183

Halvorsen S. W., 1989, Testosterone regulates aromatase activity in discrete brain areas of male rhesus macaques, Biol. Reprod., 40, 929, 10.1095/biolreprod40.5.929

10.1095/biolreprod37.3.628

10.1126/science.157.3785.201

10.1159/000125149

10.1095/biolreprod40.2.268

Schumacher M., 1986, Testosterone‐induced brain aromatase is sexually dimorphic, Brain Res., 370, 285, 10.1016/0006-8993(86)90483-X

10.1016/0006-8993(87)90548-8

Schumacher M., 1987, Interactions des androgènes et des oestrogènes dans le contrôle de la reproduction, C. R. Acad. Sci. Paris, Serie III, 305, 569

10.1210/edrv-4-2-171

10.1038/292345a0

Steimer Th. (1988). Aromatase activity in rat brain synaptosomes. Is an enzyme associated with the neuronal cell membrane involved in mediating non‐genomic effects of androgens.European J. Neurosci. Suppl.p.9(Abstract 7.5).

Steimer Th. Arsenijevic Y. Aubert M. L. andSizonenko P. C.(1989). Effects of neonatal gonadectomy and hormone treatment on testosterone metabolism in the preoptic area of male and female adult rats. Abstr. Int. Conf. Hormones Brain and Behavior Liège155–156. (Abstract).

10.1016/0022-4731(80)90286-1

10.1016/0304-3940(86)90087-X

10.1152/ajpendo.1981.241.3.E246

Thông tin
Thông tin xuất bản

Wiley

Tập 22 Số 2

143-157

Thông tin tác giả