Neonatal outcomes after the transfer of vitrified blastocysts: closed versus open vitrification system
Tóm tắt
Increasing evidence indicates that closed vitrification has been successfully used in the cryopreservation of human oocytes and embryos. Little information is available regarding the neonatal outcome of closed blastocysts vitrification. The aim of this study was to evaluate the effectiveness and safety of blastocyst vitrification using a high-security closed vitrification system compared with an open vitrification system. A total of 332 vitrified-warmed blastocyst transfer cycles between April 2010 and May 2012 were analyzed retrospectively. The post-thaw survival rate, implantation rate, clinical pregnancy rate, live birth rate, and neonatal outcome were recorded. There were no significant differences between the open vitrification group and the close vitrification group regarding the post-thaw survival rate (98% versus 95.8%), clinical pregnancy rate (47.6% versus 42.2%), implantation rate (42.9% versus 35.6%), and live birth rate (39.8% versus 32.1%). In total, 332 warming cycles produced 131 healthy babies. There were no significant differences in the mean gestational age, the birth weight, and the birth length between the two groups. No adverse neonatal outcomes were observed in the children born after the transfer of closed vitrified blastocysts compared with the transfer of open vitrified blastocysts. These data suggest that blastocyst vitrification using a closed vitrification device seems safe and effective with results comparable to those obtained through open vitrification.
Tài liệu tham khảo
Loutradi KE, Kolibianakis EM, Venetis CA, Papanikolaou EG, Pados G, Bontis I, Tarlatzis BC: Cryopreservation of human embryos by vitrification or slow freezing: a systematic review and meta-analysis. Fertil Steril. 2008, 1: 186-193.
Takahashi K, Mukaida T, Goto T, Oka C: Perinatal outcome of blastocyst transfer with vitrification using cryoloop: a 4-year follow-up study. Fertil Steril. 2005, 84: 88-92. 10.1016/j.fertnstert.2004.12.051.
Bielanski A, Naditi-Davis S, Sapp T, Lutze-Wallace C: Viral contamination of embryos cryopreserved in liquid nilrogen. Cryobiology. 2000, 40: 110-116. 10.1006/cryo.1999.2227.
Bielanski A, Vajta G: Risk of contamination of germplasm during cryopreservation and cryobanking in IVF units. Hum Reprod. 2009, 24: 2457-2467. 10.1093/humrep/dep117.
Bielanski A, Bergeron H, Lau P, Devenish J: Microbial contamination of embryos and semen during long term banking in liquid nitrogen. Cryobiology. 2003, 46: 146-152. 10.1016/S0011-2240(03)00020-8.
Kuwayama M, Vajta G, Ieda S, Kato O: Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination. Reprod Biomed Online. 2005, 11: 608-614. 10.1016/S1472-6483(10)61169-8.
Mukaida T, Takahashi K, Kasai M: Blastocyst cryopreservation: ultrarapid vitrification using cryoloop technique. Reprod Biomed Online. 2003, 6: 221-225. 10.1016/S1472-6483(10)61713-0.
Vanderzwalmen P, Ectors F, Grobet L, Prapas Y, Panagiotidis Y, Vanderzwalmen S, Stecher A, Frias P, Liebermann J, Zech NH: Aseptic vitrification of blastocysts from infertile patients, egg donors and after IVM. Reprod Biomed Online. 2009, 19: 700-707. 10.1016/j.rbmo.2009.09.011.
Van Landuyt L, Stoop D, Verheyen G, Verpoest W, Camus M, Van de Velde H, Devroey P, Van den Abbeel E: Outcome of closed blastocyst vitrification in relation to blastocyst quality: evaluation of 759 warming cycles in a single-embryo transfer policy. Hum Reprod. 2011, 26: 527-534. 10.1093/humrep/deq374.
Van Landuyt L, Verpoest W, Verheyen G, De Vos A, Van de Velde H, Liebaers I, Devroey P, Van den Abbeel E: Closed blastocyst vitrification of biopsied embryos: evaluation of 100 consecutive warming cycles. Hum Reprod. 2011, 26: 316-322. 10.1093/humrep/deq338.
AbdelHafez F, Jing X, Goldberg J, Desai N: Vitrification in open and closed carriers at different cell stages: assessment of embryo survival, development, DNA integrity and stability during vapor phase storage for transport. BMC Biotechnol. 2011, 11: 29-49. 10.1186/1472-6750-11-29.
Gardner DK, Schoolcraft WB: In vitro culture of human blastocyst. Towards Reproductive Certainty: Infertility and Genetics Beyond. Edited by: Jansen R, Mortimer D. 1999, Carnforth: Parthenon Press, 378-388.
Mukaida T, Nakamura S, Tomiyama T, Wada S, Oka C, Kasai M, Takahashi K: Vitrification of human blastocysts using cryoloops: clinical outcome of 223 cycles. Hum Reprod. 2003, 18: 384-391. 10.1093/humrep/deg047.
Seki S, Mazur P: The dominance of warming rate over cooling rate in the survival of mouse oocytes subjected to a vitrification procedure. Cryobiology. 2009, 59: 75-82. 10.1016/j.cryobiol.2009.04.012.
Panagiotidis Y, Vanderzwalmen P, Prapas Y, Kasapi E, Goudakou M, Papatheodorou A, Passadaki T, Petousis S, Nikolettos N, Veletza S, et al: Open versus closed vitrification of blastocysts from an oocyte-donation programme: a prospective randomized study. Reprod Biomed Online. 2013, 26: 470-476. 10.1016/j.rbmo.2013.01.016.
Mukaida T, Oka C, Goto T, Takahashi K: Artificial shrinkage of blastocoeles using either a micro-needle or a laser pulse prior to the cooling steps of vitrification improves survival rate and pregnancy outcome of vitrified human blastocysts. Hum Reprod. 2006, 21: 3246-3252. 10.1093/humrep/del285.
Maheshwari A, Pandey S, Shetty A, Hamilton M, Bhattacharya S: Obstetric and perinatal outcomes in singleton pregnancies resulting from the transfer of frozen thawed versus fresh embryos generated through in vitro fertilization treatment: a systematic review and meta-analysis. Fertil Steril. 2012, 2: 368-377.
Edgar DH, Gook DA: A critical appraisal of cryopreservation (slow cooling versus vitrification) of human oocytes and embryos. Hum Reprod Update. 2012, 18: 536-554. 10.1093/humupd/dms016.
Wang XL, Zhang X, Qin YQ, Hao DY, Shi HR: Outcomes of day 3 embryo transfer with vitrification using Cryoleaf: a 3-year follow-up study. J Assist Reprod Genet. 2012, 29: 883-889. 10.1007/s10815-012-9814-y.
Wikland M, Hardarson T, Hillensjö T, Westin C, Westlander G, Wood M, Wennerholm UB: Obstetric outcomes after transfer of vitrified blastocysts. Hum Reprod. 2010, 25: 1699-1707. 10.1093/humrep/deq117.
Wirleitner B, Vanderzwalmen P, Schwerda D, Bach M, Stecher A, Zech NH: No impact of long-term storage on aseptically vitrified blastocysts-pregnancy rate, life birth rate and health of children. Fertil Steril. 2012, 98: S57-S58.