Negative staining and image classification — powerful tools in modern electron microscopy

Springer Science and Business Media LLC - Tập 6 Số 1 - Trang 23-34 - 2004
Melanie D. Ohi1, Ying Liu2, Yifan Cheng1, Thomas Walz1
1Department of Cell Biology, Harvard Medical School, Boston, USA
2Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, USA

Tóm tắt

Từ khóa


Tài liệu tham khảo

Radermacher M, Wagenknecht T, Verschoor A, Frank J. Three-dimensional reconstruction from a single-exposure, random conical tilt series applied to the 50S ribosomal subunit of Escherichia coli. J Microsc 1987; 146:113–136.

Adrian M, Dubochet J, Lepault J, McDowall AW. Cryoelectron microscopy of viruses. Nature 1984; 308:32–36.

Orlova EV, Rahman MA, Gowen B, Volynski KE, Ashton AC, Manser C, van Heel M, Ushkaryov YA. Structure of alpha-latrotoxin oligomers reveals that divalent cation-dependent tetramers form membrane pores. Nat Struct Biol 2000; 7:48–53.

Stark H, Dube P, Luhrmann R, Kastner B. Arrangement of RNA and proteins in the spliceosomal U1 small nuclear ribonucleoprotein particle. Nature 2001; 409:539–542.

Cheng Y, Zak O, Aisen P, Harrison SC, Walz T. Structure of the human transferrin receptor-transferrin complex. Cell 2004; 116:565–576.

Van Heel M. Angular reconstitution: a posteriori assignment of projection directions for 3D reconstruction. Ultramicroscopy 1987; 21:111–123.

Lederkremer GZ, Cheng Y, Petre BM, Vogan E, Springer S, Schekman R, Walz T, Kirchhausen T. Structure of the Sec23p/24p and Sec13p/31p complexes of COPII. Proc Natl Acad Sci USA 2001; 98:10704–10709.

Toth EA, Li Y, Sawaya MR, Cheng Y, Ellenberger T. The crystal structure of the bifunctional primase-helicase of bacteriophage T7. Mol Cell 2003; 12:1113–1123.

Takagi J, Petre BM, Walz T, Springer TA. Global conformational rearrangements in integrin extracellular domains in outside-in and inside-out signaling. Cell 2002; 110:599–611.

Takagi J, Strokovich K, Springer TA, Walz T. Structure of integrin α5β1 in complex with fibronectin. EMBO J 2003; 22:4607–4615.

Leggett DS, Hanna J, Borodovsky A, Crosas B, Schmidt M, Baker RT, Walz T, Ploegh H, Finley D. Multiple associated proteins regulate proteasome structure and function. Mol Cell 2002; 10:495–507.

Cascio P, Call M, Petre BM, Walz T, Goldberg AL. Properties of the hybrid form of the 26S proteasome containing both 19S and PA28 complexes. EMBO J 2002; 21:2636–2645.

Walz T, Jamieson SJ, Bowers CM, Bullough PA, Hunter CN. Projection structures of three photosynthetic complexes from Rhodobacter sphaeroides: LH2 at 6 Å, LH1 and RC-LH1 at 25 Å. J Mol Biol 1998; 282:833–845.

Frank J, Radermacher M, Penczek P, Zhu J, Li Y, Ladjadj M, Leith A. SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields. J Struct Biol 1996; 116:190–199.

Adrian M, Dubochet J, Fuller SD, Harris JR. Cryo-negative staining. Micron 1998; 29:145–160.

Golas MM, Sander B, Will CL, Lurhmann R, Stark H. Molecular architecture of the multiprotein splicing factor SF3b. Science 2003; 300:980–984.

Bremer A, Henn C, Engel A, Baumeister W, Aebi U. Has negative staining still a place in biomacromolecular electron microscopy? Ultramicroscopy 1992; 46:85–111.

Baumeister W, Dahlmann B, Hegerl R, Kopp F, Kuehn L, Pfeifer G. Electron microscopy and image analysis of the multicatalytic proteinase. FEBS Lett 1988; 241:239–245.

Brink J, Van Breemen JF, Keegstra W, Van Bruggen EF. Computer image analysis of two-dimensional crystals of beef heart NADH: ubiquinone oxidoreductase fragments. I. Comparison of crystal structures in various negative stains. Ultramicroscopy 1989; 27:79–90.

Walz T, Haner M, Wu XR, Henn C, Engel A, Sun TT, Aebi U. Towards the molecular architecture of the asymmetric unit membrane of the mammalian urinary bladder epithelium: a closed “twisted ribbon” structure. J Mol Biol 1995; 248:887–900.

Zhao FQ, Craig R. Capturing time-resolved changes in molecular structure by negative staining. J Struct Biol 2003; 141:43–52.

Frank J. Three-Dimensional Electron Microscopy of Macromolecular Assemblies. 1st ed. San Diego: Academic Press, Inc.; 1996.

Samso M, Palumbo MJ, Radermacher M, Liu JS, Lawrence CE. A Bayesian method for classification of images from electron micrographs. J Struct Biol 2002; 138:157–170.

van Heel M, Harauz G, Orlova EV, Schmidt R, Schatz M. A new generation of the IMAGIC image processing system. J Struct Biol 1996; 116:17–24.

Ludtke SJ, Baldwin PR, Chiu W. EMAN: semiautomated software for high-resolution single-particle reconstructions. J Struct Biol 1999; 128:82–97.

Groll M, Ditzel L, Lowe J, Stock D, Bochtler M, Bartunik HD, Huber R. Structure of 20S proteasome from yeast at 2.4 Å resolution. Nature 1997; 386:463–471.

Bi X, Corpina RA, Goldberg J. Structure of the Sec23/24-Sar1 pre-budding complex of the COPII vesicle coat. Nature 2002; 419:271–277.

Adams GM, Crotchett B, Slaughter CA, DeMartino GN, Gogol EP. Formation of proteasome-PA700 complexes directly correlates with activation of peptidase activity. Biochemistry 1998; 37:12927–12932.

Stauffer KA, Hoenger A, Engel A. Two-dimensional crystals of Escherichia coli maltoporin and their interaction with the maltose-binding protein. J Mol Biol 1992; 223:1155–1165.

Stahlberg H, Dubochet J, Vogel H, Ghosh R. Are the light-harvesting I complexes from Rhodospirillum rubrum arranged around the reaction centre in a square geometry? J Mol Biol 1998; 282:819–831.

Tahara Y, Ohnishi S, Fujiyoshi Y, Kimura Y, Hayashi Y. A pH induced two-dimensional crystal of membrane-bound Na+,K+-ATPase of dog kidney. FEBS Lett 1993; 320:17–22.

Tahara Y, Oshima A, Hirai T, Mitsuoka K, Fujiyoshi Y, Hayashi Y. The 11 Å resolution projection map of Na+/K+-ATPase calculated by application of single particle analysis to two-dimensional crystal images. J Electron Microsc 2000; 49:583–587.

Xiong JP, Stehle T, Diefenbach B, Zhang R, Dunker R, Scott DL, Joachimiak A, Goodman SL, Arnaout MA. Crystal structure of the extracellular segment of integrin αvβ3. Science 2001; 294:339–345.

Leahy DJ, Aukhil I, Erickson HP. 2.0 Å crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region. Cell 1996; 84:155–164.