Nanoemulsion of eucalyptus oil and its larvicidal activity against Culex quinquefasciatus

Bulletin of Entomological Research - Tập 104 Số 3 - Trang 393-402 - 2014
Saranya Sugumar1, S.K. Clarke1, M. Joyce Nirmala1, B. K. Tyagi2, Amitava Mukherjee1, Natarajan Chandrasekaran1
1Centre for Nanobiotechnology, VIT University, Vellore, India
2Centre for Research in Medical Entomology, Madurai, India

Tóm tắt

AbstractFilariasis is a mosquito-borne disease that causes lymphedema and the main vector is Culex quinquefasciatus. A simple measure was taken to eradicate the vector using nanoemulsion. Eucalyptus oil nanoemulsion was formulated in various ratios comprising of eucalyptus oil, tween 80 and water by ultrasonication. The stability of nanoemulsion was observed over a period of time and 1:2 ratios of eucalyptus oil (6%) and surfactant (12%) was found to be stable. The formulated eucalyptus oil nanoemulsion was characterized by transmission electron microscopy and dynamic light scattering. The nanoemulsion droplets were found to have a Z-average diameter of 9.4 nm and were spherical in shape. The larvicidal activity of eucalyptus oil nanoemulsion and bulk emulsion was tested and compared. Our nanoemulsion showed higher activity when compared to bulk emulsion. The histopathology of larvae-treated and untreated nanoemulsion was analyzed. Furthermore, biochemical assays were carried out to examine the effect of nanoemulsion on biochemical characteristics of larvae. The treated larval homogenate showed decrease in total protein content and a significant reduction in the levels of acetylcholinesterase. The levels of acid and alkaline phosphatase also showed reduction as compared to control larval homogenate.

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Tài liệu tham khảo

10.1016/j.foodchem.2011.11.091

10.1016/j.cocis.2008.01.002

10.1016/S0021-9797(69)80012-3

Phasomkusolsil, 2010, Potential larvicidal and pupacidal activities of herbal essential oils against Culex quinquefasciatus Say and Anopheles minimus (Theobald), The Southeast Asian Journal of Tropical Medicine and Public Health, 41, 1342

10.3201/eid0404.980410

10.1016/j.actatropica.2012.01.015

10.1016/j.jcis.2007.04.079

Trigg, 1996, Evaluation of eucalyptus-based repellent against Anopheles spp. in Tanzania, Journal of the American Mosquito Control Association, 12, 243

10.1603/0022-2585-41.4.726

10.1016/0076-6879(81)71086-3

10.1016/0022-0981(78)90067-9

10.1016/j.micron.2011.07.014

Brooker, 1990, Field Guide to the Eucalyptus, 1

10.1016/j.biortech.2007.04.066

10.1007/s11356-012-1161-0

10.1016/0003-2697(76)90527-3

Global Health (2010). Division of parasitic Diseases and Malaria. “About Malaria: Malaria” 8 February 2010. CDC, Global Health – Division of Parasitic Diseases. 08 May 2010. http://www.cdc.gov/malaria/about/index.html

10.1016/j.pestbp.2006.12.004

10.1016/j.ijpharm.2004.05.016

10.1603/0022-2585-39.6.882

Wiseman, 2005, Larvicidal effects of aqueous extracts of Balanites aegyptiaca (desert date) against the larvae of Culex pipiens mosquitoes, African Journal of Biotechnology, 4, 1351

10.1111/j.1750-3841.2007.00507.x

10.1016/j.ejpb.2004.11.009

10.1007/s101560170022

Wirth, 1999, Selection and characterization of temephos resistance in a population of Aedes aegypti from Tortola, British Virgin Islands, Journal of the American Mosquito Control Association, 15, 315

10.1002/ps.2233

10.1186/1475-2875-10-S1-S11

10.1016/S2221-1691(12)60372-4

10.1016/j.pestbp.2012.09.008

10.1016/j.jspr.2003.07.002

10.1021/jf200450m

10.1016/j.jksus.2010.02.004

10.1248/cpb.56.1097

10.1590/S0074-02762003000500020

10.1021/jf200094m

10.1007/978-1-4757-5897-9

10.1016/S1369-5266(02)00251-0

McClements, 2005, Food Emulsions: Principles, Practice, and Techniques

Torres M. & Forman H.J. (2006) Signal transduction. Encyclopedia of Respiratory Medicine, pp. 10–18.

10.1021/jf050709v

10.1111/j.1750-3841.2009.01457.x

10.1016/j.actatropica.2011.01.003

10.1111/j.1365-2915.2011.00945.x

10.1016/j.ejpb.2006.10.014

Elbanna, 2006, Larvaecidal effects of eucalyptus extract on the larvae of Culex pipiens mosquito, International Journal of Agriculture and Biology, 8, 896

10.1016/j.jaad.2007.10.005

10.1016/j.ijpharm.2007.04.027

10.1016/S2221-1691(11)60135-4

10.1016/j.cbpa.2006.01.020

10.1016/j.ecoenv.2010.08.039

Sukumar, 1991, Botanical derivatives in mosquito-control – a review, Journal of the American Mosquito Control Association, 7, 210

10.1016/B978-0-12-091302-2.50067-0

10.1016/j.foreco.2008.08.008

10.1016/S0378-8741(01)00384-1

10.1016/j.jcis.2009.11.046

10.1016/j.cis.2003.10.023

WHO (2005) Guidelines for laboratory and field testing of mosquito larvicides, CDS/WHOPES/GCDPP/05.13.

10.1016/j.biortech.2007.05.064

10.1016/S0261-2194(00)00079-X