NMR Metabolite Profiles of the Bivalve Mollusc Mytilus galloprovincialis Before and After Immune Stimulation With Vibrio splendidus

Andree, 2021, Vibrio mediterranei, a Potential Emerging Pathogen of marine Fauna: Investigation of Pathogenicity Using a Bacterial challenge in Pinna nobilis and Development of a Species‐Specific PCR, J. Appl. Microbiol., 130, 617, 10.1111/jam.14756

Aru, 2020, First-Principles Identification of C-Methyl-Scyllo-Inositol (Mytilitol) - A New Species-Specific Metabolite Indicator of Geographic Origin for marine Bivalve Molluscs (Mytilus and Ruditapes spp), Food Chem., 328, 126959, 10.1016/j.foodchem.2020.126959

Aru, 2016, Metabolomics Analysis of Shucked Mussels' Freshness, Food Chem., 205, 58, 10.1016/j.foodchem.2016.02.152

Balbi, 2013, Interactions between Mytilus galloprovincialis Hemocytes and the Bivalve Pathogens Vibrio aestuarianus 01/032 and Vibrio splendidus LGP32, Fish Shellfish Immunol., 35, 1906, 10.1016/j.fsi.2013.09.027

Balseiro, 2011, Mytilus galloprovincialis Myticin C: A Chemotactic Molecule with Antiviral Activity and Immunoregulatory Properties, PLOS ONE, 6, e23140, 10.1371/journal.pone.0023140

Beirnaert, 2018, Speaq 2.0: A Complete Workflow for High-Throughput 1D NMR Spectra Processing and Quantification, Plos Comput. Biol., 14, e1006018, 10.1371/journal.pcbi.1006018

Bortoletto, 2021, Evolutionary Insights on a Novel Mussel-Specific Foot Protein-3α Gene Family, INVERT SURVIV J., 18, 19, 10.25431/1824-307X/isj.v18i1.19-32

Burton, 2005, Quantitative1H NMR with External Standards: Use in Preparation of Calibration Solutions for Algal Toxins and Other Natural Products, Anal. Chem., 77, 3123, 10.1021/ac048385h

Cappello, 2018, Baseline Levels of Metabolites in Different Tissues of Mussel Mytilus galloprovincialis (Bivalvia: Mytilidae), Comp. Biochem. Physiol. D: Genomics Proteomics, 26, 32, 10.1016/j.cbd.2018.03.005

Cappello, 2020, NMR-Based Metabolomics of Aquatic Organisms, Encyclopedia of Magnetic Resonance, 81

Cellura, 2007, Specific expression of antimicrobial peptide and HSP70 genes in response to heat-shock and several bacterial challenges in mussels, Fish Shellfish Immunol., 22, 340, 10.1016/j.fsi.2006.06.007

Collicutt, 1977, The Anaerobic Oyster Heart: Coupling of Glucose and Aspartate Fermentation, J. Comp. Physiol. B, 115, 147, 10.1007/BF00692526

Conway, 2017, UpSetR: An R Package for the Visualization of Intersecting Sets and Their Properties, Bioinformatics, 33, 2938, 10.1093/bioinformatics/btx364

Crook, 2020, Quantitative NMR-Based Biomedical Metabolomics: Current Status and Applications, Molecules, 25, 5128, 10.3390/molecules25215128

De Zwaan, 1992, Chapter 6: Cellular Biochemistry and Endocrinology, The Mussel, 223

De Zwaan, 1976, Anaerobic Metabolism in Bivalvia (Mollusca) Characteristics of Anaerobic Metabolism, Comp. Biochem. Physiol. B: Comp. Biochem., 54, 313, 10.1016/0305-0491(76)90247-9

Digilio, 2016, Haemolymph from Mytilus galloprovincialis: Response to Copper and Temperature Challenges Studied by 1H-NMR Metabonomics, Comp. Biochem. Physiol. C: Toxicol. Pharmacol., 61, 10.1016/j.cbpc.2016.02.003

Fernández Robledo, 2019, From the Raw Bar to the Bench: Bivalves as Models for Human Health, Develop. Comp. Immunol., 92, 260, 10.1016/j.dci.2018.11.020

Folch, 1957, A Simple Method for the Isolation and Purification of Total Lipides from Animal Tissues, J. Biol. Chem., 226, 497, 10.1016/S0021-9258(18)64849-5

François, 2015, Effects of a Municipal Effluent on the Freshwater Mussel Elliptio complanata Following challenge with Vibrio anguillarum, J. Environ. Sci., 37, 91, 10.1016/j.jes.2015.03.029

Garcia, 2021, Vibrio aestuarianus Subsp. cardii Subsp. nov., Pathogenic to the Edible Cockles Cerastoderma edule in France, and Establishment of Vibrio aestuarianus Subsp. aestuarianus Subsp. Nov. and Vibrio aestuarianus Subsp. francensis Subsp. Nov, Int. J. Syst. Evol. Microbiol., 71, 004654, 10.1099/ijsem.0.004654

Gerdol, 2018, Immunity in Molluscs: Recognition and Effector Mechanisms, with a Focus on Bivalvia, Advances in Comparative Immunology, 225, 10.1007/978-3-319-76768-0_11

Gerdol, 2020, Massive Gene Presence-Absence Variation Shapes an Open Pan-Genome in the Mediterranean Mussel, Genome Biol., 21, 275, 10.1186/s13059-020-02180-3

Green, 2019, Simulated Marine Heat Wave Alters Abundance and Structure of Vibrio Populations Associated with the Pacific Oyster Resulting in a Mass Mortality Event, Microb. Ecol., 77, 736, 10.1007/s00248-018-1242-9

Hao, 2014, Bayesian Deconvolution and Quantification of Metabolites in Complex 1D NMR Spectra Using BATMAN, Nat. Protoc., 9, 1416, 10.1038/nprot.2014.090

Hawkins, 1991, Protein Turnover: A Functional Appraisal, Funct. Ecol., 5, 222, 10.2307/2389260

Kong, 2020, Transcriptome and Metabolome Integration Analysis of Mud Crab Scylla paramamosain Challenged to Vibrio parahaemolyticus Infection, Fish Shellfish Immunol., 103, 430, 10.1016/j.fsi.2020.05.069

Kuhn, 2015, Facilitating Quality Control for Spectra Assignments of Small Organic Molecules: Nmrshiftdb2 - a Free In-House NMR Database with Integrated LIMS for Academic Service Laboratories, Magn. Reson. Chem., 53, 582, 10.1002/mrc.4263

Le Roux, 2009, Genome Sequence of Vibrio splendidus: An Abundant Planctonic marine Species with a Large Genotypic Diversity, Environ. Microbiol., 11, 1959, 10.1111/j.1462-2920.2009.01918.x

Li, 2020, An Integrated Omics Approach to Investigate Summer Mortality of New Zealand Greenshell Mussels, Metabolomics, 16, 100, 10.1007/s11306-020-01722-x

Liu, 2013, Differential Metabolic Responses of Clam Ruditapes philippinarum to Vibrio anguillarum and Vibrio splendidus Challenges, Fish Shellfish Immunol., 35, 2001, 10.1016/j.fsi.2013.09.014

Moreira, 2020, Stimulation of Mytilus galloprovincialis Hemocytes with Different Immune Challenges Induces Differential Transcriptomic, miRNomic, and Functional Responses, Front. Immunol., 11, 606102, 10.3389/fimmu.2020.606102

Mustafa, 1983, Gluconeogenesis in Facultative Anaerobic Invertebrates: Evidence of Oxalacetate Decarboxylation and Anaerobic End Product Incorporation into Glycogen from the Tissues ofTubifex Sp, J. Comp. Physiol. B, 149, 477, 10.1007/BF00690006

Nagana Gowda, 2019, Overview of NMR Spectroscopy-Based Metabolomics: Opportunities and Challenges, NMR-Based Metabolomics: Methods and Protocols Methods in Molecular Biology, 3, 10.1007/978-1-4939-9690-2_1

Nguyen, 2018, Metabolic and Immunological Responses of Male and Female new Zealand Greenshell Mussels (Perna canaliculus) Infected with Vibrio Sp, J. Invertebr. Pathol., 157, 80, 10.1016/j.jip.2018.08.008

Nicastro, 2010, The Role of Gaping Behaviour in Habitat Partitioning Between Coexisting Intertidal Mussels, BMC Ecol., 10, 17, 10.1186/1472-6785-10-17

Pang, 2020, MetaboAnalystR 3.0: Toward an Optimized Workflow for Global Metabolomics, Metabolites, 10, 186, 10.3390/metabo10050186

Parisi, 2008, Differential Involvement of Mussel Hemocyte Sub-Populations in the Clearance of Bacteria, Fish Shellfish Immunol., 25, 834, 10.1016/j.fsi.2008.09.005

Parisi, 2019, Responses of marine Mussel Mytilus galloprovincialis (Bivalvia: Mytilidae) After Infection with the Pathogen Vibrio splendidus, Comp. Biochem. Physiol. Part C: Toxicol. Pharmacol., 221, 1, 10.1016/j.cbpc.2019.03.005

Petronini, 1992, Modulation by Betaine of Cellular Responses to Osmotic Stress, Biochem. J., 282, 69, 10.1042/bj2820069

Poirier, 2014, Antimicrobial Histones and DNA Traps in Invertebrate Immunity, J. Biol. Chem., 289, 24821, 10.1074/jbc.M114.576546

Rey-Campos, , Immune Tolerance in Mytilus galloprovincialis Hemocytes After Repeated Contact with Vibrio splendidus, Front. Immunol., 10, 1894, 10.3389/fimmu.2019.01894

Rey-Campos, , High Individual Variability in the Transcriptomic Response of Mediterranean Mussels to Vibrio Reveals the Involvement of Myticins in Tissue Injury, Sci. Rep., 9, 3569, 10.1038/s41598-019-39870-3

Romero, 2014, Occurrence, Seasonality and Infectivity of Vibrio Strains in Natural Populations of Mussels Mytilus galloprovincialis, Dis. Aquat. Org., 108, 149, 10.3354/dao02701

Romero, 2020, Extracellular Traps (ETosis) Can Be Activated Through NADPH-Dependent and -Independent Mechanisms in Bivalve Mollusks, Develop. Comp. Immunol., 106, 103585, 10.1016/j.dci.2019.103585

Roques, 2020, Proton-NMR Metabolomics of Rainbow Trout Fed a Plant-Based Diet Supplemented with Graded Levels of a Protein-Rich Yeast Fraction Reveal Several Metabolic Processes Involved in Growth, J. Nutr., 150, 2268, 10.1093/jn/nxaa206

Rosani, 2021, Digging into Bivalve miRNAomes: Between Conservation and Innovation, Phil. Trans. R. Soc. B, 376, 20200165, 10.1098/rstb.2020.0165

Rosato, 2018, From Correlation to Causation: Analysis of Metabolomics Data Using Systems Biology Approaches, Metabolomics, 14, 37, 10.1007/s11306-018-1335-y

Ross, 2020, Proteome Turnover in the Spotlight: Approaches, Applications, and Perspectives, Mol. Cell Proteomics, 20, 100016, 10.1074/mcp.R120.002190

Saco, 2020, Transcriptomic Response of Mussel Gills After a Vibrio splendidus Infection Demonstrates Their Role in the Immune Response, Front. Immunol., 11, 3273, 10.3389/fimmu.2020.615580

Sendra, 2020, Immune-Responsive Gene 1 (IRG1) and Dimethyl Itaconate Are Involved in the Mussel Immune Response, Fish Shellfish Immunol., 106, 645, 10.1016/j.fsi.2020.07.034

Sun, 2014, The Protein Expression Profile in Hepatopancreas of Scallop Chlamys farreri under Heat Stress and Vibrio anguillarum challenge, Fish Shellfish Immunol., 36, 252, 10.1016/j.fsi.2013.11.008

Takeuchi, 2016, Bivalve-Specific Gene Expansion in the Pearl Oyster Genome: Implications of Adaptation to a Sessile Lifestyle, Zoolog. Lett, 2, 3, 10.1186/s40851-016-0039-2

Tanguy, 2018, The Immune Response of Mytilus edulis Hemocytes Exposed to Vibrio splendidus LGP32 Strain: A Transcriptomic Attempt at Identifying Molecular Actors, Fish Shellfish Immunol., 74, 268, 10.1016/j.fsi.2017.12.038

Tikunov, 2010, Metabolomic Investigations of American Oysters Using 1H-NMR Spectroscopy, Mar. Drugs, 8, 2578, 10.3390/md8102578

Ulrich, 2008, BioMagResBank, Nucleic Acids Res., 36, D402, 10.1093/nar/gkm957

Van den Berg, 2006, Centering, Scaling, and Transformations: Improving the Biological Information Content of Metabolomics Data, BMC Genomics, 7, 142, 10.1186/1471-2164-7-142

Venier, 2011, Insights into the Innate Immunity of the Mediterranean Mussel Mytilus galloprovincialis, BMC Genomics, 12, 69, 10.1186/1471-2164-12-69

Venter, 2021, Establishing Sampling Confidence Parameters: Effect of Sampling and Transport Conditions on Haemocyte and Metabolite Profiles of Greenshell Mussels, Aquaculture, 538, 736538, 10.1016/j.aquaculture.2021.736538

Vezzulli, 2010, Vibrio Infections Triggering Mass Mortality Events in a Warming Mediterranean Sea, Environ. Microbiol., 12, 2007, 10.1111/j.1462-2920.2010.02209.x

Watanabe, 2015, Application of NMR-Based Metabolomics for Environmental Assessment in the Great Lakes Using Zebra Mussel (Dreissena polymorpha), Metabolomics, 11, 1302, 10.1007/s11306-015-0789-4

Wijsman, 2019, Global Production of Marine Bivalves. Trends and Challenges, Goods and Services of Marine Bivalves, 7, 10.1007/978-3-319-96776-9_2

Wishart, 2018, HMDB 4.0: the Human Metabolome Database for 2018, Nucleic Acids Res., 46, D608, 10.1093/nar/gkx1089

Zurburg, 1980, Organ Specific Changes in Energy Metabolism Due to Anaerobiosis in the Sea Mussel Mytilus edulis (L.), Comp. Biochem. Physiol. Part B: Comp. Biochem., 67, 317, 10.1016/0305-0491(80)90150-9