NG2 glial cells integrate synaptic input in global and dendritic calcium signals

eLife - Tập 5
Wenjing Sun1, Elizabeth Matthews1, Vicky Nicolas1, Susanne Schoch2, Dirk Dietrich1
1Department of Neurosurgery, University Clinic Bonn, Bonn, Germany;
2Department of Neuropathology, University Clinic Bonn, Bonn, Germany

Tóm tắt

Synaptic signaling to NG2-expressing oligodendrocyte precursor cells (NG2 cells) could be key to rendering myelination of axons dependent on neuronal activity, but it has remained unclear whether NG2 glial cells integrate and respond to synaptic input. Here we show that NG2 cells perform linear integration of glutamatergic synaptic inputs and respond with increasing dendritic calcium elevations. Synaptic activity induces rapid Ca2+ signals mediated by low-voltage activated Ca2+ channels under strict inhibitory control of voltage-gated A-type K+ channels. Ca2+ signals can be global and originate throughout the cell. However, voltage-gated channels are also found in thin dendrites which act as compartmentalized processing units and generate local calcium transients. Taken together, the activity-dependent control of Ca2+ signals by A-type channels and the global versus local signaling domains make intracellular Ca2+ in NG2 cells a prime signaling molecule to transform neurotransmitter release into activity-dependent myelination.

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Tài liệu tham khảo

Allbritton, 1992, Range of messenger action of calcium ion and inositol 1,4,5-trisphosphate, Science, 258, 1812, 10.1126/science.1465619

Almeida, 2014, On the resemblance of synapse formation and CNS myelination, Neuroscience, 276, 98, 10.1016/j.neuroscience.2013.08.062

Bergles, 2000, Glutamatergic synapses on oligodendrocyte precursor cells in the hippocampus, Nature, 405, 187, 10.1038/35012083

Butt, 2005, Synantocytes: the fifth element, Journal of Anatomy, 207, 695, 10.1111/j.1469-7580.2005.00458.x

Chan, 2013, Ba2+- and bupivacaine-sensitive background K+ conductances mediate rapid EPSP attenuation in oligodendrocyte precursor cells, The Journal of Physiology, 591, 4843, 10.1113/jphysiol.2013.257113

Cheli, 2015, Voltage-gated Ca2+ entry promotes oligodendrocyte progenitor cell maturation and myelination in vitro, Experimental Neurology, 265, 69, 10.1016/j.expneurol.2014.12.012

Chittajallu, 2004, NG2-positive cells in the mouse white and grey matter display distinct physiological properties, The Journal of Physiology, 561, 109, 10.1113/jphysiol.2004.074252

Choe, 2011, TTA-P2 is a potent and selective blocker of T-type calcium channels in rat sensory neurons and a novel antinociceptive agent, Molecular Pharmacology, 80, 900, 10.1124/mol.111.073205

Clarke, 2012, Properties and fate of oligodendrocyte progenitor cells in the corpus callosum, motor cortex, and piriform cortex of the mouse, Journal of Neuroscience, 32, 8173, 10.1523/JNEUROSCI.0928-12.2012

Clements, 1997, Detection of spontaneous synaptic events with an optimally scaled template, Biophysical Journal, 73, 220, 10.1016/S0006-3495(97)78062-7

De Biase, 2010, Excitability and synaptic communication within the oligodendrocyte lineage, Journal of Neuroscience, 30, 3600, 10.1523/JNEUROSCI.6000-09.2010

Gabso, 1997, Low mobility of the Ca2+ buffers in axons of cultured Aplysia neurons, Neuron, 18, 473, 10.1016/S0896-6273(00)81247-7

Gallo, 1996, Oligodendrocyte progenitor cell proliferation and lineage progression are regulated by glutamate receptor-mediated K+ channel block, Journal of Neuroscience, 16, 2659, 10.1016/0736-5748(96)80263-2

Ge, 2006, Long-term potentiation of neuron-glia synapses mediated by Ca2+-permeable AMPA receptors, Science, 312, 1533, 10.1126/science.1124669

Ge, 2009, Dividing glial cells maintain differentiated properties including complex morphology and functional synapses, PNAS, 106, 328, 10.1073/pnas.0811353106

Ghiani, 1999, Neurotransmitter receptor activation triggers p27(Kip1 )and p21(CIP1) accumulation and G1 cell cycle arrest in oligodendrocyte progenitors, Development, 126, 1077, 10.1242/dev.126.5.1077

Ghiani, 1999, Voltage-activated K+ channels and membrane depolarization regulate accumulation of the cyclin-dependent kinase inhibitors p27(Kip1) and p21(CIP1) in glial progenitor cells, Journal of Neuroscience, 19, 5380, 10.1523/JNEUROSCI.19-13-05380.1999

Haberlandt, 2011, Gray matter NG2 cells display multiple Ca2+-signaling pathways and highly motile processes, PLoS One, 6, e17575, 10.1371/journal.pone.0017575

Helmchen, 1996, Ca2+ buffering and action potential-evoked Ca2+ signaling in dendrites of pyramidal neurons, Biophysical Journal, 70, 1069, 10.1016/S0006-3495(96)79653-4

Hines, 2015, Neuronal activity biases axon selection for myelination in vivo, Nature Neuroscience, 18, 683, 10.1038/nn.3992

Iwamoto, 1996, A novel isothiourea derivative selectively inhibits the reverse mode of Na+/Ca2+ exchange in cells expressing NCX1, The Journal of Biological Chemistry, 271, 22391, 10.1074/jbc.271.37.22391

Jabs, 2005, Synaptic transmission onto hippocampal glial cells with hGFAP promoter activity, Journal of Cell Science, 118, 3791, 10.1242/jcs.02515

Káradóttir, 2008, Spiking and nonspiking classes of oligodendrocyte precursor glia in CNS white matter, Nature Neuroscience, 11, 450, 10.1038/nn2060

Knutson, 1997, K+ channel expression and cell proliferation are regulated by intracellular sodium and membrane depolarization in oligodendrocyte progenitor cells, Journal of Neuroscience, 17, 2669, 10.1523/JNEUROSCI.17-08-02669.1997

Koudelka, 2016, Individual neuronal subtypes exhibit diversity in CNS myelination mediated by synaptic vesicle release, Current Biology, 26, 1447, 10.1016/j.cub.2016.03.070

Kukley, 2007, Vesicular glutamate release from axons in white matter, Nature Neuroscience, 10, 311, 10.1038/nn1850

Kukley, 2008, Glial cells are born with synapses, The FASEB Journal, 22, 2957, 10.1096/fj.07-090985

Kukley, 2010, The fate of synaptic input to NG2 glial cells: neurons specifically downregulate transmitter release onto differentiating oligodendroglial cells, Journal of Neuroscience, 30, 8320, 10.1523/JNEUROSCI.0854-10.2010

Larson, 2016, Electrophysiological properties of NG2(+) cells: Matching physiological studies with gene expression profiles, Brain Research, 1638, 138, 10.1016/j.brainres.2015.09.010

Lin, 2004, Synaptic signaling between GABAergic interneurons and oligodendrocyte precursor cells in the hippocampus, Nature Neuroscience, 7, 24, 10.1038/nn1162

Lin, 2005, Climbing fiber innervation of NG2-expressing glia in the mammalian cerebellum, Neuron, 46, 773, 10.1016/j.neuron.2005.04.025

Lytle, 2009, NG2 cell response in the CNP-EGFP mouse after contusive spinal cord injury, Glia, 57, 270, 10.1002/glia.20755

Matthews, 2013, Tuning local calcium availability: cell-type-specific immobile calcium buffer capacity in hippocampal neurons, Journal of Neuroscience, 33, 14431, 10.1523/JNEUROSCI.4118-12.2013

Mensch, 2015, Synaptic vesicle release regulates myelin sheath number of individual oligodendrocytes in vivo, Nature Neuroscience, 18, 628, 10.1038/nn.3991

Newcomb, 1998, Selective peptide antagonist of the class E calcium channel from the venom of the tarantula Hysterocrates gigas, Biochemistry, 37, 15353, 10.1021/bi981255g

Nishiyama, 1999, NG2+ glial cells: a novel glial cell population in the adult brain, Journal of Neuropathology and Experimental Neurology, 58, 1113, 10.1097/00005072-199911000-00001

Paez, 2009, Voltage-operated Ca(2+) and Na(+) channels in the oligodendrocyte lineage, Journal of Neuroscience Research, 87, 3259, 10.1002/jnr.21938

Paez, 2010, Multiple kinase pathways regulate voltage-dependent Ca2+ influx and migration in oligodendrocyte precursor cells, Journal of Neuroscience, 30, 6422, 10.1523/JNEUROSCI.5086-09.2010

Passlick, 2016, The NG2 protein is not required for glutamatergic neuron-NG2 cell synaptic signaling, Cerebral Cortex, 26, 51, 10.1093/cercor/bhu171

Pende, 1994, Glutamate regulates intracellular calcium and gene expression in oligodendrocyte progenitors through the activation of DL-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors, PNAS, 91, 3215, 10.1073/pnas.91.8.3215

Petersen, 2015, Neurobiology: Myelin goes where the action is, Current Biology, 25, R562, 10.1016/j.cub.2015.04.054

Pivonkova, 2010, Impact of global cerebral ischemia on K+ channel expression and membrane properties of glial cells in the rat hippocampus, Neurochemistry International, 57, 783, 10.1016/j.neuint.2010.08.016

Steinhäser, 1994, Properties of GABA and glutamate responses in identified glial cells of the mouse hippocampal slice, Hippocampus, 4, 19, 10.1002/hipo.450040105

Steinhäuser, 1994, Properties of voltage-activated Na+ and K+ currents in mouse hippocampal glial cells in situ and after acute isolation from tissue slices, Pflügers Archiv European Journal of Physiology, 428, 610, 10.1007/BF00374585

Sun, 2013, Synaptic integration by NG2 cells, Frontiers in Cellular Neuroscience, 7, 255, 10.3389/fncel.2013.00255

Tong, 2009, Ca(2+) signaling evoked by activation of Na(+) channels and Na(+)/Ca(2+) exchangers is required for GABA-induced NG2 cell migration, The Journal of Cell Biology, 186, 113, 10.1083/jcb.200811071

Trotter, 2010, NG2 cells: Properties, progeny and origin, Brain Research Reviews, 63, 72, 10.1016/j.brainresrev.2009.12.006

Vélez-Fort, 2010, Postnatal switch from synaptic to extrasynaptic transmission between interneurons and NG2 cells, Journal of Neuroscience, 30, 6921, 10.1523/JNEUROSCI.0238-10.2010

Wagner, 1994, Effects of rapid buffers on Ca2+ diffusion and Ca2+ oscillations, Biophysical Journal, 67, 447, 10.1016/S0006-3495(94)80500-4

Wake, 2011, Control of local protein synthesis and initial events in myelination by action potentials, Science, 333, 1647, 10.1126/science.1206998

Zhou, 1993, Mobile and immobile calcium buffers in bovine adrenal chromaffin cells, The Journal of Physiology, 469, 245, 10.1113/jphysiol.1993.sp019813

Zhu, 2008, NG2 cells generate both oligodendrocytes and gray matter astrocytes, Development, 135, 145, 10.1242/dev.004895

Ziskin, 2007, Vesicular release of glutamate from unmyelinated axons in white matter, Nature Neuroscience, 10, 321, 10.1038/nn1854