Mycobacterium tuberculosis Lineage Distribution in Xinjiang and Gansu Provinces, China

Scientific Reports - Tập 7 Số 1
Haixia Chen1, Li He2, Hairong Huang3,4, Cheng‐Min Shi5, Xumin Ni6, Guangming Dai3,4, Liang Ma5, Weimin Li7
1Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, 325035, China
2Academy of Mathematics and Systems Science, Chinese Academy of Sciences, Beijing 100190, China
3Beijing Chest Hospital, Capital Medical University, Beijing 101149, China
4National Tuberculosis Clinical Lab of China, Beijing Tuberculosis and Thoracic Tumor Research Institute
5Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China
6Department of Mathematics, School of Science, Beijing Jiaotong University, Beijing 100044, China
7Beijing Key Laboratory in Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing, 101149, China

Tóm tắt

Abstract

Mycobacterium tuberculosis (M. tuberculosis) genotyping has dramatically improved the understanding of the epidemiology of tuberculosis (TB). In this study, 187 M. tuberculosis isolates from Xinjiang Uygur Autonomous Region (Xinjiang) and Gansu province in China were genotyped using large sequence polymorphisms (LSPs) and variable number tandem repeats (VNTR). Ten isolates, which represent major nodes of VNTR-based minimum spanning tree, were selected and subsequently subjected to multi-locus sequence analyses (MLSA) that include 82 genes. Based on a robust lineage assignment, we tested the association between lineages and clinical characteristics by logistic regression. There are three major lineages of M. tuberculosis prevalent in Xinjiang, viz. the East Asian Lineage 2 (42.1%; 56/133), the Euro-American Lineage 4 (33.1%; 44/133), and the Indian and East African Lineage 3 (24.8%; 33/133); two lineages prevalent in Gansu province, which are the Lineage 2 (87%; 47/54) and the Lineage 4 (13%; 7/54). The topological structures of the MLSA-based phylogeny support the LSP-based identification of M. tuberculosis lineages. The statistical results suggest an association between the Lineage 2 and the hemoptysis/bloody sputum symptom, fever in Uygur patients. The pathogenicity of the Lineage 2 remains to be further investigated.

Từ khóa


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Tập 7 Số 1

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