Multisite Comparison of High-Sensitivity Multiplex Cytokine Assays

American Society for Microbiology - Tập 18 Số 8 - Trang 1229-1242 - 2011
Elizabeth C. Breen1,2, S. R. M. Reynolds3, Christopher Cox3, Lisa P. Jacobson3, Larry Magpantay4, Candice Mulder5, Oliver Dibben6, Joseph B. Margolick7, Jay H. Bream7, Elise Sambrano8, Otoniel Martı́nez-Maza9,10,4, Elizabeth Sinclair8, Persephone Borrow6, Alan Landay5, Charles R. Rinaldo11, Philip J. Norris12,13,14
1Departments of Psychiatry and Biobehavioral Sciences, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, California
2UCLA Cousins Center for Psychoneuroimmunology, Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California, Los Angeles, CA 90095-7076, USA.
3Departments of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
4Obstetrics and Gynecology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, California
5Rush University Medical Center, Chicago, Illinois
6Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom
7Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland
8Division of Experimental Medicine, University of California, San Francisco, California
9Department of Epidemiology, School of Public Health, University of California, Los Angeles, California
10Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, California
11Department of Infectious Diseases and Microbiology, University of Pittsburgh Graduate School of Public Health, Pittsburgh, Pennsylvania
12Blood Systems Research Institute, San Francisco, California
13Departments of Medicine, University of California, San Francisco, California
14Laboratory Medicine, University of California, San Francisco, California

Tóm tắt

ABSTRACT The concentrations of cytokines in human serum and plasma can provide valuable information about in vivo immune status, but low concentrations often require high-sensitivity assays to permit detection. The recent development of multiplex assays, which can measure multiple cytokines in one small sample, holds great promise, especially for studies in which limited volumes of stored serum or plasma are available. Four high-sensitivity cytokine multiplex assays on a Luminex (Bio-Rad, BioSource, Linco) or electrochemiluminescence (Meso Scale Discovery) platform were evaluated for their ability to detect circulating concentrations of 13 cytokines, as well as for laboratory and lot variability. Assays were performed in six different laboratories utilizing archived serum from HIV-uninfected and -infected subjects from the Multicenter AIDS Cohort Study (MACS) and the Women's Interagency HIV Study (WIHS) and commercial plasma samples spanning initial HIV viremia. In a majority of serum samples, interleukin-6 (IL-6), IL-8, IL-10, and tumor necrosis factor alpha were detectable with at least three kits, while IL-1β was clearly detected with only one kit. No single multiplex panel detected all cytokines, and there were highly significant differences ( P < 0.001) between laboratories and/or lots with all kits. Nevertheless, the kits generally detected similar patterns of cytokine perturbation during primary HIV viremia. This multisite comparison suggests that current multiplex assays vary in their ability to measure serum and/or plasma concentrations of cytokines and may not be sufficiently reproducible for repeated determinations over a long-term study or in multiple laboratories but may be useful for longitudinal studies in which relative, rather than absolute, changes in cytokines are important.

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American Society for Microbiology

Tập 18 Số 8

1229-1242

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