Multiresistance Genes of Rhizobium etli CFN42

Molecular Plant-Microbe Interactions - Tập 13 Số 5 - Trang 572-577 - 2000
Ramón González-Pasayo1, Esperanza Martı́nez-Romero2,3,4
1Centro de Investigación sobre Fijación de Nitrógeno, and Facultad de Ciencias, Universidad Nacional Autónoma de México, Cuernavaca, Morelos.
2Address: Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Apartado Postal 565-A, Cuernavaca, Morelos, México
3Fax: (52) (73) 17 55 81
4Telephone: (52) (73) 13 16 97

Tóm tắt

Multidrug efflux pumps of bacteria are involved in the resistance to various antibiotics and toxic compounds. In Rhizobium etli, a mutualistic symbiont of Phaseolus vulgaris (bean), genes resembling multidrug efflux pump genes were identified and designated rmrA and rmrB. rmrA was obtained after the screening of transposon-generated fusions that are inducible by bean-root released flavonoids. The predicted gene products of rmrAB shared significant homology to membrane fusion and major facilitator proteins, respectively. Mutants of rmrA formed on average 40% less nodules in bean, while mutants of rmrA and rmrB had enhanced sensitivity to phytoalexins, flavonoids, and salicylic acid, compared with the wild-type strain. Multidrug resistance genes emrAB from Escherichia coli complemented an rmrA mutant from R. etli for resistance to high concentrations of naringenin.

Từ khóa


Tài liệu tham khảo

10.1016/S0022-2836(05)80360-2

10.1146/annurev.ge.29.120195.000543

10.1128/jb.179.2.364-369.1997

10.1128/jb.174.16.5183-5189.1992

10.1128/jb.171.1.8-15.1989

10.1046/j.1365-2958.1998.00657.x

10.1094/MPMI-6-665

10.1086/414909

Eisenschenk L., 1994, Appl. Environ. Microbiol., 60, 3315, 10.1128/aem.60.9.3315-3322.1994

10.1099/00221287-16-2-374

10.1016/0378-1119(82)90167-6

10.1016/0955-0674(92)90090-Y

Hanin, M., Jabbouri, S., Broughton, W. J., Fellay, R., and Quesada-Vincens, D. 1999. Molecular aspects of host-specific nodulation. Pages 1-37 in: Plant-Microbe Interactions. Vol. 4. G. Stacey and N. T. Keen, eds. American Phytopathological Society, St. Paul, MN.

10.1073/pnas.89.22.10915

10.1126/science.8153629

10.1006/fgbi.1998.1046

10.1104/pp.97.2.759

10.1016/0378-1119(87)90193-4

10.1007/BF00016484

10.1146/annurev.py.33.090195.001423

10.1016/0022-2836(82)90515-0

10.1016/0968-0004(94)90204-6

10.1093/nar/21.7.1507

10.1073/pnas.89.19.8938

10.1016/0378-1119(94)90327-1

10.1016/0968-0004(93)90081-W

10.1094/MPMI.1998.11.2.153

10.1016/0076-6879(83)01005-8

10.1002/prot.340110203

10.1007/BF00196971

10.1128/JB.180.12.3107-3113.1998

Pao S. S., 1998, Microbiol. Mol. Biol. Rev., 62, 1, 10.1128/MMBR.62.1.1-34.1998

10.1128/jb.173.11.3432-3439.1991

10.1016/0378-1119(93)90755-R

10.1046/j.1365-2958.1999.01361.x

10.1128/jb.176.17.5409-5413.1994

10.1007/s004380050772

10.1073/pnas.83.6.1757

10.1111/j.1365-2958.1990.tb00565.x

10.1096/fasebj.12.3.265

10.1111/j.1365-2958.1994.tb00362.x

Sambrook, J., Fritsch, E. F., and Maniatis, T. A. 1989. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

10.1094/MPMI-7-0384

10.1099/00207713-43-2-374

10.1007/BF00182799

10.1038/nbt1183-784

10.1016/0378-1119(89)90262-X

10.1146/annurev.py.33.090195.002021

10.1515/znc-1990-9-1006

Thông tin
Thông tin xuất bản

Molecular Plant-Microbe Interactions

Tập 13 Số 5

572-577

Thông tin tác giả