Multiple metabotropic glutamate receptors regulate hippocampal function

Synapse - Tập 12 Số 3 - Trang 206-213 - 1992
Manisha Desai1, Timothy S. Smith1, P. Jeffrey Conn1
1Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia 30322

Tóm tắt

AbstractSelective activation of metabotropic glutamate receptors withtrans‐1‐amino‐1, 3‐cyclopentanedicarboxylic acid (trans‐ACPD) stimulates phosphoinositide hydrolysis and elicits three major physiological responses in area CA1 of the hippocampus. These include direct excitation of pyramidal cells, blockade of synaptic inhibition, and decreased transmission at the Schaffer collateral‐CA1 pyramidal cell synapse. Physiological effects oftrans‐ACPD are thought to be mediated by activation of phosphoinositide hydrolysis. However, it is now clear that multiple metabotropic glutamate receptor subtypes exist, some of which are not coupled to phosphoinositide hydrolysis. Thus, we performed a series of studies aimed at determining whether the physiological effects oftrans‐ACPD in the hippocampus are mediated by activation of the predominant phosphoinositide hydrolysis‐linked glutamate receptor. We report thatL‐2‐amino‐3‐phosphonopropionic acid (L‐AP3), an antagonist oftrans‐ACPD‐stimulated phosphoinositide hydrolysis, does not inhibit the physiological effects oftrans‐ACPD in area CA1 at concentrations that maximally inhibittrans‐ACPD‐stimulated phosphoinositide hydrolysis in this region. Furthermore, 1S, 3S‐ACPD activates the phosphoinositide hydrolysis‐linked glutamate receptor but does not reduce evoked field excitatory postsynaptic potentials (EPSPs) in area CA1. However, we report that the physiological effects of 1R, 3S‐ and 1S, 3R‐ACPD are consistent with the hypothesis that these effects are mediated by activation of a metabotropic glutamate receptor. Thus, our data are consistent with the hypothesis that the physiological effects oftrans‐ACPD in area CA1 of the hippocampus are mediated by metabotropic glutamate receptors that are distinct from the AP3‐sensitive phosphoinositide hydrolysis‐linked glutamate receptor. © 1992 Wiley‐Liss, Inc.

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Synapse

Tập 12 Số 3

206-213

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