Multiple association analysis of loci and candidate genes that regulate body size at three growth stages in Simmental beef cattle

BMC Genetics - Tập 21 - Trang 1-11 - 2020
Bingxing An, Lei Xu, Jiangwei Xia1, Xiaoqiao Wang2, Jian Miao2, Tianpeng Chang2, Meihua Song3, Junqing Ni4, Lingyang Xu2, Lupei Zhang2, Junya Li2, Huijiang Gao2
1Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, China
2Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, China
3Zhuang Yuan Veterinary Station of Qixia city, Yantai, China
4Heibei Livestock Breeding Workstation, Shijiazhuang, China

Tóm tắt

Body size traits as one of the main breeding selection criteria was widely used to monitor cattle growth and to evaluate the selection response. In this study, body size was defined as body height (BH), body length (BL), hip height (HH), heart size (HS), abdominal size (AS), and cannon bone size (CS). We performed genome-wide association studies (GWAS) of these traits over the course of three growth stages (6, 12 and 18 months after birth) using three statistical models, single-trait GWAS, multi-trait GWAS and LONG-GWAS. The Illumina Bovine HD 770 K BeadChip was used to identify genomic single nucleotide polymorphisms (SNPs) in 1217 individuals. In total, 19, 29, and 10 significant SNPs were identified by the three models, respectively. Among these, 21 genes were promising candidate genes, including SOX2, SNRPD1, RASGEF1B, EFNA5, PTBP1, SNX9, SV2C, PKDCC, SYNDIG1, AKR1E2, and PRIM2 identified by single-trait analysis; SLC37A1, LAP3, PCDH7, MANEA, and LHCGR identified by multi-trait analysis; and P2RY1, MPZL1, LINGO2, CMIP, and WSCD1 identified by LONG-GWAS. Multiple association analysis was performed for six growth traits at each growth stage. These findings offer valuable insights for the further investigation of potential genetic mechanism of growth traits in Simmental beef cattle.

Tài liệu tham khảo

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