Multilocus Sequence Typing for Characterization of Methicillin-Resistant and Methicillin-Susceptible Clones of<i>Staphylococcus aureus</i>

Journal of Clinical Microbiology - Tập 38 Số 3 - Trang 1008-1015 - 2000
Mark C. Enright1, Nicholas Day2, Catrin E. Moore2, Sharon J. Peacock2,3, Brian G. Spratt1
1<!--label omitted: 1-->Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, Oxford University, Oxford OX1 3FY,1 and
2<!--label omitted: 2-->Wellcome Trust Centre for Clinical Tropical Medicine, Nuffield Department of Medicine,2 and
3<!--label omitted: 3-->Department of Pathology and Bacteriology,3 Oxford University, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom

Tóm tắt

ABSTRACTA multilocus sequence typing (MLST) scheme has been developed forStaphylococcus aureus. The sequences of internal fragments of seven housekeeping genes were obtained for 155S. aureusisolates from patients with community-acquired and hospital-acquired invasive disease in the Oxford, United Kingdom, area. Fifty-three different allelic profiles were identified, and 17 of these were represented by at least two isolates. The MLST scheme was highly discriminatory and was validated by showing that pairs of isolates with the same allelic profile produced very similarSmaI restriction fragment patterns by pulsed-field gel electrophoresis. All 22 isolates with the most prevalent allelic profile were methicillin-resistantS. aureus(MRSA) isolates and had allelic profiles identical to that of a reference strain of the epidemic MRSA clone 16 (EMRSA-16). Four MRSA isolates that were identical in allelic profile to the other major epidemic MRSA clone prevalent in British hospitals (clone EMRSA-15) were also identified. The majority of isolates (81%) were methicillin-susceptibleS. aureus(MSSA) isolates, and seven MSSA clones included five or more isolates. Three of the MSSA clones included at least five isolates from patients with community-acquired invasive disease and may represent virulent clones with an increased ability to cause disease in otherwise healthy individuals. The most prevalent MSSA clone (17 isolates) was very closely related to EMRSA-16, and the success of the latter clone at causing disease in hospitals may be due to its emergence from a virulent MSSA clone that was already a major cause of invasive disease in both the community and hospital settings. MLST provides an unambiguous method for assigning MRSA and MSSA isolates to known clones or assigning them as novel clones via the Internet.

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Tài liệu tham khảo

Anonymous Epidemic methicillin-resistant Staphylococcus aureus.Commun. Dis. Rep. Weekly719971

10.1128/jcm.33.3.551-555.1995

10.1099/00222615-44-3-179

10.1016/0195-6701(95)90191-4

10.1089/mdr.1996.2.343

10.1128/JCM.36.9.2590-2596.1998

10.1128/jcm.32.9.2081-2087.1994

10.1128/CMR.6.4.428

10.1099/00221287-144-11-3049

10.1128/JCM.37.10.3210-3216.1999

10.1093/oxfordjournals.molbev.a026061

10.1097/00013542-199807000-00004

10.1093/jac/40.1.135

10.1017/S0950268800048901

10.1093/nar/2.3.383

10.1073/pnas.95.6.3140

10.1128/jcm.30.8.2058-2063.1992

National Committee for Clinical Laboratory Standards Performance standards for antimicrobial susceptibility testing. Ninth informational supplement. 1999 National Committee for Clinical Laboratory Standards Villanova Pa

10.1128/JCM.36.2.414-420.1998

Panlilio A. L. Culver D. H. Gaynes R. P. Bannerjee S. Henderson T. S. Tolson J. S. Martone W. J. Methicillin-resistant Staphylococcus aureus in US hospitals, 1975–1991.Infect. Control Hosp. Epidemiol.131992582586

10.1016/0195-6701(93)90007-M

10.1086/513758

Sawyer S. Statistical tests for gene conversion.Mol. Biol. Evol.61989526538

10.1099/00222615-47-4-341

10.1128/JCM.36.12.3514-3519.1998

10.1056/NEJM199902183400701

10.1016/S0140-6736(97)12148-1

10.1016/S1369-5274(99)80054-X

10.1093/clinids/23.2.255

Streulens M. J. R. Bax A. Deplano W. G. V. Quint and A. van Belkum. Concordant clonal delineation of methicillin-resistant Staphylococcus aureus by macrorestriction analysis and polymerase chain reaction genome fingerprinting. J. Clin. Microbiol. 31: 1964–1970.

10.1128/JCM.33.9.2233-2239.1995

10.1128/AAC.36.1.6

10.1016/S0195-6701(97)90056-6

10.1128/JCM.36.6.1653-1659.1998

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Journal of Clinical Microbiology

Tập 38 Số 3

1008-1015

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