Multicolor Spectral Karyotyping of Human Chromosomes

American Association for the Advancement of Science (AAAS) - Tập 273 Số 5274 - Trang 494-497 - 1996
Evelin Schröck1, Stanislas du Manoir1, Timothy Veldman1, Brigitte Schoell1, Johannes Wienberg2, M.A. Ferguson‐Smith2, Yi Ning1, David H. Ledbetter1, Irit Bar‐Am3, Dirk Soenksen4, Yuval Garini5, Thomas Ried1
1E. Schröck, S. du Manoir, T. Veldman, B. Schoell, Y. Ning, D. H. Ledbetter, T. Ried, Diagnostic Development Branch, National Center for Human Genome Research, National Institutes of Health, 49 Convent Drive, Building 49, Room 4A28, Bethesda, MD 20892-4470, USA.
2J. Wienberg and M. A. Ferguson-Smith, Department of Pathology, Cambridge University, Cambridge CB21QP, UK.
3I. Bar-Am, Department of Cell Research and Immunology, Tel-Aviv University, Tel Aviv 69978, Israel.
4D. Soenksen, Applied Spectral Imaging, Incorporated, Carlsbad, CA 92009, USA.
5Y. Garini, Applied Spectral Imaging, Limited, Migdal Haemek, 10551 Israel.

Tóm tắt

The simultaneous and unequivocal discernment of all human chromosomes in different colors would be of significant clinical and biologic importance. Whole-genome scanning by spectral karyotyping allowed instantaneous visualization of defined emission spectra for each human chromosome after fluorescence in situ hybridization. By means of computer separation (classification) of spectra, spectrally overlapping chromosome-specific DNA probes could be resolved, and all human chromosomes were simultaneously identified.

Từ khóa


Tài liệu tham khảo

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Thông tin xuất bản

American Association for the Advancement of Science (AAAS)

Tập 273 Số 5274

494-497

Thông tin tác giả