Multicenter comparison of serologic assays and estimation of human herpesvirus 8 seroprevalence among US blood donors

Transfusion - Tập 43 Số 9 - Trang 1260-1268 - 2003
Philip E. Pellett1, Deborah Wright2,3,4,5,6,7,8,9,10, Eric A. Engels2,3,4,5,6,7,8,9,10, Dharam V. Ablashi2,3,4,5,6,7,8,9,10, Sheila C. Dollard2,3,4,5,6,7,8,9,10, Bagher Forghani2,3,4,5,6,7,8,9,10, Sharon A. Glynn2,3,4,5,6,7,8,9,10, James J. Goedert2,3,4,5,6,7,8,9,10, Frank J. Jenkins2,3,4,5,6,7,8,9,10, T.‐H. Lee2,3,4,5,6,7,8,9,10, Frank Neipel2,3,4,5,6,7,8,9,10, Deborah Todd2,3,4,5,6,7,8,9,10, Denise Whitby2,3,4,5,6,7,8,9,10, George J. Nemo2,3,4,5,6,7,8,9,10, Michael P. Busch2,3,4,5,6,7,8,9,10
1[Cleveland Clinic Foundation]
2ABI, Columbia, Maryland
3From the Centers for Disease Control and Prevention, Atlanta, Georgia
4REDS, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland.
5Westat, Rockville, Maryland
6the Blood Centers of the Pacific and the University of California, San Francisco, California
7the Institute for Virology, University of Erlangen, Erlangen, Germany
8the National Cancer Institute, National Institutes of Health, Rockville and Frederick, Maryland
9The University of Pittsburgh, Pittsburgh, Pennsylvania
10the Viral and Rickettsial Disease Laboratory, California Department of Health Services, Richmond, California

Tóm tắt

BACKGROUND:  As part of assessing the possibility of transfusion transmission of human herpesvirus 8 (HHV‐8 or Kaposi's sarcoma‐associated herpesvirus), HHV‐8 seroprevalence was estimated among US blood donors, the performance of HHV‐8 serologic tests was compared, and the presence of HHV‐8 DNA was tested for in donated blood.STUDY DESIGN AND METHODS:  Replicate panels of 1040 plasma specimens prepared from 1000 US blood donors (collected in 1994 and 1995) and 21 Kaposi's sarcoma patients were tested for antibodies to HHV‐8 in six laboratories. HHV‐8 PCR was performed on blood samples from 138 donors, including all 33 who tested seropositive in at least two laboratories and 22 who tested positive in at least one.RESULTS:  The estimated HHV‐8 seroprevalence among US blood donors was 3.5 percent (95% CI, 1.2%‐9.8%) by a conditional dependence latent‐class model, 3.0 percent (95% CI, 2.0%‐4.6%) by a conditional independence latent‐class model, and 3.3 percent (95% CI, 2.3%‐4.6%) by use of a consensus‐derived gold standard (specimens positive in two or more laboratories); the conditional dependence model best fit the data. In this model, laboratory specificities ranged from 96.6 to 100 percent. Sensitivities ranged widely, but with overlapping 95 percent CIs. HHV‐8 DNA was detected in blood from none of 138 donors evaluated.CONCLUSIONS:  Medical and behavioral screening does not eliminate HHV‐8‐seropositive persons from the US blood donor pool, but no viral DNA was found in donor blood. Further studies of much larger numbers of seropositive individuals will be required to more completely assess the rate of viremia and possibility of HHV‐8 transfusion transmission. Current data do not indicate a need to screen US blood donors for HHV‐8.

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Transfusion

Tập 43 Số 9

1260-1268

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