Morphological effects and tegumental alterations induced by mefloquine on schistosomula and adult flukes ofSchistosoma mansoni

Parasitology - Tập 137 Số 1 - Trang 85-98 - 2010
Theresia Manneck1, Yolanda Haggenmüller1, Jennifer Keiser1
1Department of Medical Parasitology and Infection Biology, Swiss Tropical Institute, CH-4002 Basel, Switzerland

Tóm tắt

SUMMARYThere is a pressing need to develop novel anti-schistosomal drugs, as current treatment relies largely on praziquantel (PZQ). To further strengthen current evidence of the anti-schistosomal properties of mefloquine (MQ), we studied the temporal effect of this compoundin vitroandin vivo, and examined alterations on the tegumental surface of schistosomula and adults ofS. mansoniby means of scanning electron microscopy (SEM). Schistosomula and adults were each incubatedin vitrousing MQ over a wide concentration range (1–100 μg/ml). In addition, mice infected with adultS. mansoniwere treated with a single oral dose of 400 mg/kg MQ, and worms were recovered 24, 48, 72, 96 and 120 h following treatment. MQ showed a rapid onset of action on schistosomulain vitro; 100 and 75 μg/ml of MQ killed schistosomula immediately; the minimal lethal and effective concentrations of MQ on schistosomula after 1 h were 25 and 5 μg/ml, respectively. Adult worms incubated with 100 and 10 μg/ml of MQ were dead after 1 h and 24 h of incubation, respectively. A hepatic shift of adult schistosomes was observed in mice already 24 h after treatment, and 120 h following treatment >98% of all worms had translocated to the liver. SEM observations revealed extensive tegumental destruction, including blebbing, shrinking and sloughing, particularly followingin vitroincubation and on the tegument of female worms.

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Parasitology

Tập 137 Số 1

85-98

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