Morphological and growth altering effects of Cisplatin in C. albicans using fluorescence microscopy
Tóm tắt
Changes in morphology and growth curve of Candida albicans in response to treatment by Cisplatin has been studied using fluorescence staining with ethidium bromide. Treatment with Cisplatin was found to markedly inhibit hyphae and ovoid growth as revealed by ethidium bromide staining of drug treated cells. These changes were concomitant with inhibitory effects on the growth curve with respect to untreated cells Presence of Cisplatin not only caused suppression in the limiting values in the growth curve, but also caused a slight left shift in the EC50 values. Some of the ovoid cells undergoing poisoning with cisplatin were found to be unusually enlarged before undergoing their natural fate thus suggesting formation of similar cytotoxic end products with DNA.
Tài liệu tham khảo
Calderone RA, Fonzi WA: Virulence factors of Candida albicans. Trends Microbiol. 2001, 9: 327-335. 10.1016/S0966-842X(01)02094-7
Wall SD, Jones B: Gastrointestinal tract in the immunocompromised host: opportunistic infections and other complications. Radiology. 1992, 185: 327-335.
Saral R: Candida and Aspergillus infections in immunocompromised patients: an overview. Rev Infect Dis. 1991, 13: 487-492.
St GV: Membrane transporters and antifungal drug resistance. Curr Drug Targets. 2000, 1: 261-284. 10.2174/1389450003349209
Miletti KE, Leibowitz MJ: Pentamidine inhibition of group I intron splicing in Candida albicans correlates with growth inhibition. Antimicrob Agents Chemother. 2000, 44: 958-966. 10.1128/AAC.44.4.958-966.2000
Chandrasekar K, Shyla JH, Malathi R: Can antitumor platinum compounds be effective against Candida albicans?--A screening assay using disk diffusion method. J Clin Microbiol. 2000, 38: 3905-
Watanabe T, Takano M, Ogasawara A, Mikami T, Kobayashi T, Watabe M, Matsumoto T: Anti-Candida activity of a new platinum derivative. Antimicrob Agents Chemother. 2000, 44: 2853-2854. 10.1128/AAC.44.10.2853-2854.2000
Sarachek A, Henderson L, Wilkens WE: Evaluation of the genotoxic spectrum of cisplatin for Candida albicans. Microbios. 1992, 72: 183-201.
Sarachek A, Henderson LA: Modification of responses of Candida albicans to cisplatin by membrane damaging antimycotic agents. Mycoses. 1991, 34: 177-182.
Moussa NM, Ghannoum MA, Whittaker PA, el Ezaby MS, Quraman S: Effects of cisplatin and two novel palladium complexes on Candida albicans. Microbios. 1990, 62: 165-178.
Bernhardt H, Knoke M, Bernhardt J: Changes in Candida albicans colonization and morphology under influence of voriconazole. Mycoses. 2003, 46: 370-374. 10.1046/j.0933-7407.2003.00908.x
Hopfer RL, Sparks SD, Cox GM: The use of flow cytometry as a tool for monitoring filament formation of fungi. Med Mycol. 2001, 39: 103-107.
Aoki S, Ito-Kuwa S, Nakamura Y, Masuhara T: Mitochondrial behaviour during the yeast-hypha transition of Candida albicans. Microbios. 1989, 60: 79-86.
Otto AM, Paddenberg R, Schubert S, Mannherz HG: Cell-cycle arrest, micronucleus formation, and cell death in growth inhibition of MCF-7 breast cancer cells by tamoxifen and cisplatin. J Cancer Res Clin Oncol. 1996, 122: 603-612. 10.1007/BF01221192