Monoclonal antibodies to early pregnancy factor perturb tumour cell growth

Clinical and Experimental Immunology - Tập 80 Số 1 - Trang 100-108 - 2008
Kathryn Quinn1, S Athanasas-Platsis1, Tien Yin Wong1, Barbara E. Rolfe1, Alice Cavanagh1, H. Craig Morton1
1Department of Surgery, University of Queensland, Royal Brisbane Hospital, Brisbane, Australia

Tóm tắt

SUMMARY The pregnancy-associated substance early pregnancy factor (EPF) has previously been reported as a product of tumours of germ cell origin. More recently EPF (or an EPF-related substance, tEPF) has also been detected in the serum of patients bearing tumours of non-germ cell origin. We report here the production of tEPF by a variety of cultured transformed and tumour cell lines, of both germ and non-germ cell origin. Antibodies specific for EPF remove all tEPF activity from tumour cell conditioned medium, tEPF production is found to be associated with cell division; tEPF is no longer detected after growth arrest or differentiation. Co-culture of tumour cells with increasing doses of anti-EPF monoclonal antibodies resulted in a significant, dose-dependent decrease in rate of cell growth and viability. Similar anti-EPF concentrations had no effect on the concanavalin A induced proliferation of mouse spleen cells. These studies suggest, therefore, that tEPF is a growth-regulated product of cultured tumour and transformed cells. These cells are also dependent upon tEPF for continued growth, i.e. tEPF is acting in the autocrine mode.

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Clinical and Experimental Immunology

Tập 80 Số 1

100-108

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