Molecularly imprinted polymer for solid‐phase extraction of ephedrine and analogs from human plasma

Journal of Separation Science - Tập 32 Số 7 - Trang 1036-1042 - 2009
Martina Lasáková1,2, Didier Thiébaut2, Pavel Jandera1, Valérie Pichon
1Department of Analytical Chemistry, University of Pardubice, Pardubice, Czech Republic
2Department of Environmental and Analytical Chemistry (UMR CNRS PECSA 7195), ESPCI ParisTech, Paris, France.

Tóm tắt

AbstractA molecularly imprinted polymer (MIP) was synthesized and evaluated to selectively extract ephedrine from human plasma. The MIP synthesis was performed in chloroform with methacrylic acid as a functional monomer and the target alkaloid as a template molecule. The resulting MIP was applied to the selective extraction of ephedrine from a pure aqueous medium. A recovery about 74% was obtained using the MIP with only 7% on the nonimprinted polymer (NIP). A very straightforward selective SPE procedure was then successfully applied to the direct extraction of ephedrine from spiked human plasma with a high extraction recovery (68%) on the MIP with no recovery on the NIP. Moreover, the MIP was used for the selective extraction of catecholamine neurotransmitters, i.e. adrenaline and noradrenaline.

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