Nhận dạng, phân loại và truy xuất nguồn gốc của vi khuẩn lam từ các hồ chứa nước ngọt
Tóm tắt
Để đánh giá tiềm năng của một số mục tiêu phân tử trong việc xác định, phân loại và truy xuất nguồn gốc của vi khuẩn lam trong các hồ chứa nước ngọt, các kỹ thuật phân tử đã được áp dụng cho 118 thể vi khuẩn lam chủ yếu được thu thập từ các hồ chứa nước ngọt ở Bồ Đào Nha và đại diện cho ba bộ vi khuẩn lam: Chroococcales (54), Oscillatoriales (15) và Nostocales (49). Các thể vi khuẩn này đã được xác định trước đó bằng các phương pháp hình thái và sau đó được đặc trưng bởi phân tích cụm phân cấp tổng hợp dựa trên hồ sơ dấu vân tay PCR của STRR và LTRR (tương tác lặp lại ngắn và dài). Các thể vi khuẩn đại diện đã được chọn từ mỗi cụm và việc xác định phân tử của chúng, ở cấp độ loài, đã được thực hiện hoặc xác nhận thông qua việc định vị filogen bằng gene 16S rRNA và
Từ khóa
#vi khuẩn lam #nước ngọt #chỉ thị phân tử #phân loại #truy xuất nguồn gốcTài liệu tham khảo
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