Molecular genetic analysis of the <i>moa</i> operon of <i>Escherichia coli</i> K‐12 required for molybdenum cofactor biosynthesis

Molecular Microbiology - Tập 8 Số 6 - Trang 1071-1081 - 1993
Stuart L. Rivers1, Elizabeth McNairn2, Francis Blasco3, Daniela Giordano3, David H. Boxer2
1Department of Biochemistry, Dundee University, UK.
2Department of Biochemistry, Medical Sciences Institute, Dundee University, Dundee DDI 4HN, UK.
3Laboratoire de Chimie Bactérienne, CNRS, BP 71, 31 chemin J. Aiguier, 13277 Marseille Cedex 9, France.

Tóm tắt

SummaryA 3.2 kb chromosomal DNA fragment which complements the defects in a series of twelve moa::Mucts insertion mutants has been sequenced. Five open reading frames (ORFs) were identified and these are arranged in a manner consistent with their forming an operon. The encoded proteins (MoaA‐MoaE) have predicted molecular weights of 37346, 18665, 17234, 8843 and 16981 respectively. Examination of subclones of the whole locus in an expression system demonstrated the predicted products. N‐terminal amino acid sequences for the moa A, B, C and E products confirmed the translational starts. Genetic analysis distinguished four classes of moa mutants corresponding to genes moaA, C, D and E. Potential promoter sequences upstream of moaA and a possible transcription termination signal have been identified. Genetic analysis of the chlA1 and chlM mutants, which have been biochemically characterized as defective in molybdopterin biosynthesis, indicates that these carry lesions in moaA and moaD respectively. The moa locus is orientated clockwise at 17.7 minutes in the chromosome.

Từ khóa


Tài liệu tham khảo

10.1146/annurev.bi.47.070178.004535

10.1111/j.1365-2958.1991.tb00764.x

10.1128/jb.169.10.4678-4685.1987

Brown G.M., 1987, Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, 521

10.1073/pnas.82.17.5720

10.1111/j.1432-1033.1981.tb06415.x

10.1111/j.1574-6968.1981.tb07612.x

10.1016/0304-4165(85)90035-2

10.1016/0167-4838(87)90281-0

10.1111/j.1365-2958.1990.tb00633.x

10.1007/BF00334254

10.1128/jb.171.1.447-455.1989

10.1016/0167-4838(83)90158-9

10.1093/nar/11.8.2237

10.1016/0378-1119(87)90489-6

10.1128/jb.170.4.1475-1487.1988

10.1128/jb.169.5.1911-1916.1987

10.1016/S0021-9258(18)91027-6

10.1016/S0021-9258(18)80016-3

10.1073/pnas.87.8.3190

10.1128/jb.169.1.110-116.1987

10.1128/jb.169.1.117-125.1987

10.1016/0092-8674(87)90503-4

10.1128/JB.172.4.2079-2087.1990

10.1111/j.1574-6968.1978.tb02841.x

Maniatis T., 1982, Molecular Cloning: A Laboratory Manual

10.1093/nar/9.2.309

10.1128/jb.155.2.793-801.1983

10.1128/jb.169.5.1853-1860.1987

Miller J.H., 1972, Experiments in Molecular Genetics

10.1007/BF00430437

10.1128/jb.170.9.4097-4102.1988

Novel G., 1973, Mutants de Escherichia coli K12 affectés pour leur croissance sur méthyl β‐D‐glucuronide: localisation du gène de structure de la β‐D‐glucuronidase (uidA), Mol Gen Genet, 20, 319, 10.1007/BF00268146

Pittard A.J., 1987, Escherichia coli and Salmonella typhimurium; Cellular and Molecular Biology, 368

10.1128/jb.171.6.3373-3378.1989

Puig J., 1967, Étude génétique dune mutation à effet pléiotrope chez l' Escherichia coli K‐12, C R Acad Sci (Paris), 264, 1507

10.1016/S0021-9258(19)50001-1

10.1007/BF00333594

10.1093/nar/12.18.7123

10.1016/0378-1119(86)90032-6

10.1073/pnas.74.12.5463

Santini C.L., 1989, Escherichia coli molybdoenzymes can be activated by protein FA from several Gram‐negative bacteria, J Gen Microbiol, 135, 3467

10.1128/jb.174.24.7934-7940.1992

10.1016/0003-2697(87)90587-2

10.1111/j.1365-2958.1992.tb02215.x

10.1073/pnas.78.3.1596

10.1016/B978-0-12-682001-0.50009-9

10.1128/MMBR.52.2.190-232.1988

Stewart V., 1982, Nitrate reductase in Escherichia coli K‐12: involvement of chlG, chtE, and chlG loci, J Bacteriol, 151, 788, 10.1128/jb.151.2.788-799.1982

10.1128/jb.121.3.759-765.1975

10.1093/nar/10.9.2971

Studier F.W., 1986, Use of bacteriophage T7 RNA polymerase to direct selective high‐level expression of cloned genes, J Mol Biol, 199, 113, 10.1016/0022-2836(86)90385-2

10.1073/pnas.82.4.1074

10.1007/BF00427140

10.1016/S0005-2728(05)80100-8

Thông tin
Thông tin xuất bản

Molecular Microbiology

Tập 8 Số 6

1071-1081

Thông tin tác giả