Molecular genetic analysis of the moa operon of Escherichia coli K‐12 required for molybdenum cofactor biosynthesis

Molecular Microbiology - Tập 8 Số 6 - Trang 1071-1081 - 1993
Stuart L. Rivers1, Elizabeth McNairn2, Francis Blasco3, Daniela Giordano3, David H. Boxer2
1Department of Biochemistry, Dundee University, UK.
2Department of Biochemistry, Medical Sciences Institute, Dundee University, Dundee DDI 4HN, UK.
3Laboratoire de Chimie Bactérienne, CNRS, BP 71, 31 chemin J. Aiguier, 13277 Marseille Cedex 9, France.

Tóm tắt

SummaryA 3.2 kb chromosomal DNA fragment which complements the defects in a series of twelve moa::Mucts insertion mutants has been sequenced. Five open reading frames (ORFs) were identified and these are arranged in a manner consistent with their forming an operon. The encoded proteins (MoaA‐MoaE) have predicted molecular weights of 37346, 18665, 17234, 8843 and 16981 respectively. Examination of subclones of the whole locus in an expression system demonstrated the predicted products. N‐terminal amino acid sequences for the moa A, B, C and E products confirmed the translational starts. Genetic analysis distinguished four classes of moa mutants corresponding to genes moaA, C, D and E. Potential promoter sequences upstream of moaA and a possible transcription termination signal have been identified. Genetic analysis of the chlA1 and chlM mutants, which have been biochemically characterized as defective in molybdopterin biosynthesis, indicates that these carry lesions in moaA and moaD respectively. The moa locus is orientated clockwise at 17.7 minutes in the chromosome.

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Molecular Microbiology

Tập 8 Số 6

1071-1081

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