Molecular cryo‐electron tomography of vitreous tissue sections: current challenges

Journal of Microscopy - Tập 235 Số 3 - Trang 293-307 - 2009
Lars Norlén1, Ozan Öktem, Ulf Skoglund
1Department of Cell and Molecular Biology (CMB), Medical Nobel Institute, Karolinska Institute, Stockholm, Sweden. [email protected]

Tóm tắt

SummaryElectron tomography of vitreous tissue sections (tissue TOVIS) allows the study of the three‐dimensional structure of molecular complexes in a near‐native cellular context. Its usage is, however, limited by an unfortunate combination of noisy and incomplete data, by a technically demanding sample preparation procedure, and by a disposition for specimen degradation during data collection. Here we outline some major challenges as experienced from the application of TOVIS to human skin. We further consider a number of practical measures as well as theoretical approaches for its future development.

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