Molecular cloning and functional characterization of MnSOD from Dunaliella salina

Journal of Basic Microbiology - Tập 54 Số 5 - Trang 438-447 - 2014
Wei‐Jen Lee1, Xin Ran Li2, Hui Xu2, Yu Cao2, Shu Han2, Yi Cao2, Dairong Qiao2
1Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu, Sichuan 610065, P. R. China.
2College of Life Science, Sichuan University Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province Chengdu Sichuan 610065, P. R. China

Tóm tắt

AbstractDunaliella salina, a unicellular green alga, has the potential to grow in hypersaline environments via one of its gene products, superoxide dismutase (SOD). The superoxide radicals (O2−) produced by environmental stresses can cause damage to cells, and SOD catalyzes the turnover of such free radicals to protect cells. In this study, the gene coding for SOD in D. salina was cloned and the product was further identified and characterized. The open reading frame of this gene was 651 bp long, encoding for 217 amino acids. According to the sequence alignment using BLAST, native polyacrylamide electrophoresis for SOD activity analysis, and atomic absorption spectroscopy analysis, this protein belongs to the manganese‐containing superoxide dismutase (MnSOD) family. Complementation analysis, performed by introducing plasmids carrying an inducible version of the D. salina gene encoding for MnSOD into an SOD‐deficient mutant of E.coli, revealed that this gene could not only complement the defects in SOD activity, but was also capable of providing a stronger tolerance to restrictive growth conditions, such as high salt and prolonged UV exposure, compared to the tolerance of wild‐type strains.

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Thông tin xuất bản

Journal of Basic Microbiology

Tập 54 Số 5

438-447

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