Molecular cloning and characterization of alpha‐class glutathione S‐transferase gene from the liver of silver carp, bighead carp, and other major chinese freshwater fishes

Journal of Biochemical and Molecular Toxicology - Tập 20 Số 3 - Trang 114-126 - 2006
Wanqin Liao1, Xu‐Fang Liang1, Lin Wang1, Lamei Lei1, Bo‐Ping Han1
1College of Life Science and Technology, Jinan University, Shipai, Guangzhou 510632, People's Republic of China.

Tóm tắt

AbstractTwo full‐length cDNAs encoding glutathione S‐transferase (GST) were cloned and sequenced from the hepatopancreas of planktivorous silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis). The silver carp and bighead carp GST cDNA were 920 and 978 bp in length, respectively, and both contained an open reading frame that encoding 223 amino acids. Partial GST cDNA sequences were also obtained from the liver of grass carp (Ctenopharyngodon idellus), crucian carp (Carassius auratu), mud carp (Cirrhinus molitorella), and tilapia (Oreochromis nilotica). All these GSTs could be classified as alpha‐class GSTs on the basis of their amino acid sequence identity with other species. The three‐dimensional structure of the silver carp GST was predicted using a computer program, and was found to fit the classical two‐domain GST structure. Using the genome walker method, a 875‐bp 5′‐flanking region of the silver carp GST gene was obtained, and several lipopolysaccharide (LPS) response elements were identified in the promoter region of the phytoplanktivorous fish GST gene, indicating that the GST gene expression of this fish might be regulated by LPS, released from the toxic blue‐green algae producing microcystins. To compare the constitutive expression level of the liver GST gene among the six freshwater fishes with completely different tolerance to microcystins, beta‐actin was used as control and the ratio GST/beta‐actin mRNA (%) was determined as 130.7 ± 6.6 (grass carp), 103.1 ± 8.9 (bighead carp), 92.6 ± 15.0 (crucian carp), 72.3 ± 7.8 (mud carp), 58.8 ± 11.5 (silver carp), and 33.6 ± 13.7 (tilapia). The constitutive expression level of the liver GST gene clearly shows that all the six freshwater fishes had a negative relationship with their tolerance to microcystins: high‐resistant fishes (phytoplanktivorous silver carp and tilapia) had the lowest tolerance to microcystins and the high‐sensitive fish (herbivorous grass carp) had the highest tolerance to microcystins. Taken together with the reciprocal relationship of constitutive and inducible liver GST expression level in some of the tested fish species to microcystin exposure, a molecular mechanism for different microcystin detoxification abilities of the warm freshwater fishes was discussed. © 2006 Wiley Periodicals, Inc. J Biochem Mol Toxicol 20:114–126, 2006; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20125

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Tài liệu tham khảo

Codd GA, 1995, Cynobacterial toxins: occurrence, properties and biological significance, Water Sci Technol, 32, 149, 10.2166/wst.1995.0177

10.1016/S0041-0101(97)00102-5

10.1080/20018091095087

10.1111/j.1440-1746.1995.tb01370.x

10.1016/S0140-6736(97)12285-1

Sivonen K, 1999, Toxic cyanobacteria in water: A guide to their public health consequence, monitoring and management, 41

10.1007/978-3-642-60682-3_38

10.1056/NEJM199803263381304

10.2216/i0031-8884-40-3-228.1

10.1016/0005-2736(90)90190-Y

10.1006/taap.1995.1192

10.1053/jhep.2000.16183

10.1080/152873901753215966

10.1002/jat.2550110112

10.1006/enrs.1998.3843

10.1016/S0304-4165(98)00107-X

10.1016/S1382-6689(00)00049-1

10.1016/S0166-445X(02)00091-7

10.1016/S0041-0101(02)00099-5

10.1146/annurev.pharmtox.45.120403.095857

10.1016/S0021-9258(18)99004-6

10.1073/pnas.89.2.668

10.1073/pnas.90.7.2965

10.3109/10409239509083491

10.1016/0167-4781(94)90223-2

10.1006/abbi.1999.1569

Melgar‐Riol MJ, 2001, Glutathione S‐transferases from rainbow trout liver and freshly isolated hepatocytes: Purification and characterization, Comp Biochem Physiol C, 128, 227

Nóvoa‐Valiñas MC, 2002, Comparative study of the purification and characterization of the cytosolic glutathione S‐transferases from two salmonid species: Atlantic salmon (Salmo salar) and brown trout (Salmo trutta), Comp Biochem Physiol C, 131, 207

10.1042/bj2920189

10.1016/j.cca.2005.02.012

10.1042/BJ20041578

10.1016/j.bcp.2004.01.024

10.1042/bj3210405

10.1100/tsw.2001.67

10.1016/j.envpol.2003.08.011

10.1093/bioinformatics/15.7.622

10.1093/nar/gkg520

10.1016/S0968-0004(00)88963-X

10.1042/bst0240274

10.1002/elps.1150181505

10.1016/S0378-1119(01)00473-5

10.1006/jmbi.1993.1376

10.1016/0022-2836(92)90692-D

10.1016/0022-2836(90)90313-B

10.1002/jbt.20033

10.1016/j.cbpc.2004.06.019

10.1021/bi991179x

10.1021/bi991482y

Miyatake S, 1991, Characterization of the mouse granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) gene promoter: Nuclear factors that interact with an element shared by three lymphokine genes—those for GM‐CSF, interleukin‐4 (IL‐4), and IL‐5, Mol Cell Biol, 11, 5894

Nimer S, 1990, The repeated sequence CATT(A/T) is required for granulocyte‐macrophage colony‐stimulating factor promoter activity, Mol Cell Biol, 10, 6084

Rao P, 1997, Colchicine down‐regulates lipopolysaccharide‐induced granulocyte‐macrophage colony‐stimulating factor production in murine macrophages, J Immunol, 159, 3531, 10.4049/jimmunol.159.7.3531

10.1074/jbc.275.18.13179

Storey KB, 1996, Oxidative stress: Animal adaptations in nature, Braz J Med Biol Res, 29, 1715

10.1016/j.biocel.2003.10.012

10.1016/j.ecoenv.2004.11.014

10.1016/S1532-0456(03)00076-0

10.1016/S0166-445X(02)00010-3

Kajak Z, 1977, Studies on food selectivity of silver carp, Hypophthalmichthys molitrix (Val.), Ekol Polska, 25, 227

Opuszynski K, 1995, Herbivorous fishes‐culture and use for weed management

10.1111/j.1095-8649.1993.tb01164.x

10.1007/BF01203845

He JW, 1997, The toxicity of microcystis aeruginosa to fish and daphnia, J Lake Sci, 9, 49, 10.18307/1997.0108

Chen JC, 1995, Studies on the toxic effects of Microcystis on fishes, Fishery Sci Inform, 22, 169

Zhang YY, 1996, Preliminary studies on the role of GSH in detoxification of microcystin‐LR in fish, Acta Hydrobiol Sinica, 20, 284

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Journal of Biochemical and Molecular Toxicology

Tập 20 Số 3

114-126

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