Molecular characterization and pathogenicity of Mycoplasma capricolum subsp. capricpolum from goats in Morocco

Springer Science and Business Media LLC - 2022
Noha Semmate1, Zaid Zouagui2, Zouhair Elkarhat1, Zahra Bamouh2, Siham Fellahi2, Noursaid Tligui2, Zineb Boumart1, Ouafaa Fassi Fihri2, Mehdi El Harrak1
1Research and Development Department, Multi-Chemical Industry, Mohammedia, Morocco
2Agronomic and Veterinary Institute Hassan II, BP: 6202, Rabat Institutions, Rabat, Morocco

Tóm tắt

AbstractMycoplasma capricolum subsp. capricolum (Mcc) is an important etiological agent of contagious agalactia (CA). CA affects small ruminants and is characterized by inducing mastitis, arthritis, kerato-conjunctivitis and respiratory symptoms. The aim of this study was to isolate and characterize Mcc from Moroccan goats with contagious agalactia. A total of 300 Alpine goats were monitored. Serology analysis, molecular identification, and isolation of Mcc were realized from suspected goats. An experimental study was conducted for isolated Mcc to determine their pathogenicity. Thus, clinical observation showed that respiratory symptoms were predominant in young animals, and other symptoms, such as mastitis, keratoconjunctivitis and lameness, were more frequently detected in adult goats. Of the 80 tested blood samples, 28 sera were seropositive for Mcc antibodies. Mcc was identified by polymerase chain reaction (PCR) in milk, lung tissue and synovial liquid samples. The isolation of Mcc was successful through bacterial culture from lung tissue. LppA gene sequence of this strain revealed 98.1% similarity with the reference strain (ATCC 27343), with 11 missense variants. Experimental infection resulted in severe and generalized CA disease in sheep and goats, confirming the high pathogenicity of the Moroccan Mcc isolate.

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