Molecular Biomarkers for Weight Control in Obese Individuals Subjected to a Multiphase Dietary Intervention

Journal of Clinical Endocrinology and Metabolism - Tập 102 Số 8 - Trang 2751-2761 - 2017
Jennifer L. Bolton1,2, Émilie Montastier1,3, Jérôme Carayol4, Sophie Bonnel1,2, Lucile Mir1,2, Marie‐Adeline Marques1,2, Arne Astrup5, Wim H. M. Saris6, Jason S. Iacovoni1,2, Nathalie Villa‐Vialaneix7, Armand Valsesia4, Dominique Langin1,3, Nathalie Viguerie1,2
1Institut National de la Santé et de la Recherche Médicale, Obesity Research Laboratory, Institute of Metabolic and Cardiovascular Diseases, 31432 Toulouse, France
2University of Toulouse, Paul Sabatier University, 31400 Toulouse, France
3Toulouse University Hospitals, Departments of Endocrinology, Metabolism and Nutrition, 31400 Toulouse, France
4Nestlé Institute of Health Sciences SA, CH-1015 Lausanne, Switzerland
5Department of Nutrition, Exercise and Sports, Faculty of Sciences, University of Copenhagen, 2200 Copenhagen, Denmark
6Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre, 6229 Maastricht, The Netherlands
7Unité de Mathématiques et Informatique Appliquées de Toulouse, Université de Toulouse, 31326 Castanet Tolosan, France

Tóm tắt

AbstractContextAlthough calorie restriction has proven beneficial for weight loss, long-term weight control is variable between individuals.ObjectiveTo identify biomarkers of successful weight control during a dietary intervention (DI).Design, Setting, and ParticipantsAdipose tissue (AT) transcriptomes were compared between 21 obese individuals who either maintained weight loss or regained weight during the DI. Results were validated on 310 individuals from the same study using quantitative reverse transcription polymerase chain reaction and protein levels of potential circulating biomarkers measured by enzyme-linked immunosorbent assay.InterventionIndividuals underwent 8 weeks of low-calorie diet, then 6 months of ad libitum diet.Outcome MeasureWeight changes at the end of the DI.ResultsWe evaluated six genes that had altered expression during DI, encode secreted proteins, and have not previously been implicated in weight control (EGFL6, FSTL3, CRYAB, TNMD, SPARC, IGFBP3), as well as genes for which baseline expression differed between those with good and poor weight control (ASPN, USP53). Changes in plasma concentrations of EGFL6, FSTL3, and CRYAB mirrored AT messenger RNA expression; all decreased during DI in individuals with good weight control. ASPN and USP53 had higher baseline expression in individuals who went on to have good weight control. Expression quantitative trait loci analysis found polymorphisms associated with expression levels of USP53 in AT. A regulatory network was identified in which transforming growth factor β1 (TGF-β1) was responsible for downregulation of certain genes during DI in good controllers. Interestingly, ASPN is a TGF-β1 inhibitor.ConclusionsWe found circulating biomarkers associated with weight control that could influence weight management strategies and genes that may be prognostic for successful weight control.

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Journal of Clinical Endocrinology and Metabolism

Tập 102 Số 8

2751-2761

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