Minimal system for assembly of SARS-CoV-2 virus like particles

Scientific Reports - Tập 10 Số 1
Heather Swann1, Abhimanyu Sharma2, Benjamin Preece1, Abby Peterson1, Crystal Eldredge1, David M. Belnap3, Michael Vershinin2, Saveez Saffarian2
1Center for Cell and Genome Science, University of Utah, Salt Lake City, USA
2[Department of Physics and Astronomy, University of Utah, Salt Lake CIty, USA]
3School of Biological Sciences, University of Utah, Salt Lake City, USA

Tóm tắt

AbstractSARS-CoV-2 virus is the causative agent of COVID-19. Here we demonstrate that non-infectious SARS-CoV-2 virus like particles (VLPs) can be assembled by co-expressing the viral proteins S, M and E in mammalian cells. The assembled SARS-CoV-2 VLPs possess S protein spikes on particle exterior, making them ideal for vaccine development. The particles range in shape from spherical to elongated with a characteristic size of 129 ± 32 nm. We further show that SARS-CoV-2 VLPs dried in ambient conditions can retain their structural integrity upon repeated scans with Atomic Force Microscopy up to a peak force of 1 nN.

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Scientific Reports

Tập 10 Số 1

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