Mimicry of snail host antigens by miracidia and primary sporocysts of Schistosoma mansoni

Parasite Immunology - Tập 5 Số 3 - Trang 317-328 - 1983
Timothy P. Yoshino1, Christopher J. Bayne2,1
1Department of Zoology, University of Oklahoma, Norman, Oklahoma 73019
2Department of Zoology, Oregon State University, Corvallis, Oregon 97331, USA

Tóm tắt

Summary Polyvalent antisera generated in rabbits to soluble haemolymph components from Schistosoma /warworn‐susceptible (PR albino ‘M line’) and S. /warworn‐resistant (10‐R2) stocks of the snail Biomphalaria glabrata were employed as membrane probes to determine if antigens related to snail haemolymph were produced by the early larval stages of S. mansoni (PR‐1 strain). Using immunofluorescence and immunoelectron microscopical methods we have demonstrated that antibodies to susceptible (anti‐Suscept) and resistant (anti‐Resist) snail haemolymph (Hg‐depleted fraction) crossreact with miracidial epidermal and ciliary membranes as well as the surface membranes of intercellular ridges. Primary sporocysts, both transformed in vitro and maintained in culture for various time intervals in the absence of snail‐derived factors, retain haemolymph‐like antigens on their surface tegument although at reduced levels in comparison to miracidial stages. Furthermore prolonged cultivation of sporocysts (48 h) has little effect on the density of crossreacting tegumental antigens suggesting that as sporocysts mature these antigenic components are continually being expressed at the surface membrane. Since miracidia and sporocysts were derived in media devoid of snail host materials, shared antigens on larval surfaces are believed to be of parasite origin and constitute true molecular mimicry as defined by Damian (1979). The occurrence of crossreacting antibodies in both anti‐Suscept and anti‐Resist antisera further suggests that mimicked haemolymph‐like antigens include at least some which are common to both snail stocks.

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Tài liệu tham khảo

10.2307/3279129

10.1016/0014-4894(76)90022-9

10.2307/3278518

Bayne C.J., 1980, Aspects of Developmental and Comparative Immunology, 115

Bayne C.J., 1983, Biology of Mollusca

10.1007/978-1-4899-2993-8_4

Capron A., 1965, Les antigenes de Schistosoma II Etude immunoelectrophoretique comparee de divers stades larvaires et des adultes des de Smansoni Annates de Institut Pasteur, Paris, 109, 798

10.1007/BF00201974

10.1007/978-1-4615-9131-3_2

10.1086/282313

Damian R.T., 1979, Host‐Parasite Interfaces, 103

10.2307/3278895

Figueiredo E.A., 1973, Isolation and physicochemical properties of the hemoglobin of Biomphalaria glabrata (Mollusca, Planorbidae), Comparative Biochemistry and Physiology, 44, 481

10.1016/0014-4894(71)90057-9

Houba V., 1976, Pathophysiology of Parasite Infection, 221

10.1017/S0022149X00028832

Kemp W.M., 1974, Sharing of cercarienhullen reaktion antigens between Schistosoma mansoni cercariae and adults and uninfected Biomphalaria pfeifferi, American Journal of Tropical Medicine and Hygiene, 23, 197, 10.4269/ajtmh.1974.23.197

Kemp W.M., 1980, Tegument‐associated antigen modulation by adult male Schistosoma mansoni, Journal of Immunology, 124, 806, 10.4049/jimmunol.124.2.806

10.1017/S0031182000000846

10.1016/0014-4894(72)90111-7

10.2307/3273773

10.1017/S0031182000049696

10.1016/0014-4894(77)90146-1

10.1111/j.1550-7408.1974.tb03718.x

10.1098/rspb.1969.0007

10.1016/0022-2011(79)90125-3

10.1016/0022-2011(79)90149-6

10.1002/9780470720073.ch5

Wright C.A., 1971, The Science of Biology

10.1016/0145-305X(81)90030-6

10.2307/3279977

10.2307/3279884

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Thông tin xuất bản

Parasite Immunology

Tập 5 Số 3

317-328

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