Microsatellites provide insight into contrasting mating patterns in arribada vs. non‐arribada olive ridley sea turtle rookeries

Molecular Ecology - Tập 15 Số 9 - Trang 2567-2575 - 2006
Michael P. Jensen1, F. Alberto Abreu‐Grobois, Jane Frydenberg, Volker Loeschcke
1Department of Biological Sciences, Ecology and Genetics, University of Aarhus, Ny Munkegade, Build. 540, DK-8000 Aarhus C, Denmark. [email protected]

Tóm tắt

AbstractMolecular studies of sea turtles have shown that the frequency of multiple paternity (MP) varies between species, and between rookeries of the same species. This study uses nuclear microsatellite markers to compare the incidence of MP in two neighbouring olive ridley rookeries on the Pacific coast of Costa Rica, with contrasting nesting behaviours — the ‘arribada’ population nesting at Ostional and the solitary nesters of Playa Hermosa. Using two highly polymorphic microsatellite markers, we tested 13 nests from each location and found a significant difference (P < 0.001) between the level of MP of the arribada rookery (92%— the highest found for marine turtles) and that of the solitary nesting rookery (30%). Additional analyses based on six microsatellite loci revealed no genetic differentiation between nesting females from the two locations, or between nesting females and attendant males from the Ostional breeding area. Sixty‐nine per cent of the nests with MP were fathered by a minimum of three different males, and three nests showed evidence of at least four fathers. The results suggest that the differences observed in levels of MP between arribada and solitary rookeries are due to an effect of abundance of individuals on the mating system. This is supported by a regression analysis combining other paternity studies on sea turtles which shows that levels of MP increase with increasing abundance of nesting females.

Từ khóa


Tài liệu tham khảo

10.1046/j.1471-8286.2003.00574.x

10.1002/jmor.1052190309

10.1038/16451

BagleyDA UongLT EhrhartLM(1998)Marine turtle nesting in the Archie Carr National Wildlife Refuge in 1996: a new record for leatherbacks. In:Proceedings of the Seventeenth International Symposium on Sea Turtle Conservation Genetics(edsEpperlySP BraunJ) pp.130–131. US Department of Commerce NOAA Technical Memorandum NMFS‐SEFSC‐415.

10.2307/1447494

Briseño‐DueñasR(1998)Variación genética de la región control del ADN mitocondrial de poblaciones de la tortuga golfina(Lepidochelys olivacea)en el Pacífico oriental e implicaciones para su conservación.Master thesis Universidad Autónoma de Sinaloa México.

Broderick AC, 2002, A Management Plan for The Marine Turtles of Ascension Island

Cornelius SE, 1991, Neotropical Wildlife Use and Conservation, 111

10.1046/j.1365-294X.2002.01591.x

DuttonPH(1996)Methods for collection and preservation of samples for sea turtle genetic studies. In:Proceedings of the International Symposium on Sea Turtle Conservation Genetics(edsBowenBW WitzellWN) pp.17–24. US Department of Commerce NOAA Technical Memorandum NMFS‐SEFSC‐396.

10.1006/anbe.1999.1212

10.2307/1437776

FitzSimmonsNN(1996)Use of microsatellite loci to investigate multiple paternity in marine turtles. In:Proceedings of the International Symposium on Sea Turtle Conservation Genetics(edsBowenBW WitzellWN) pp.69–78. US Department of Commerce NOAA Technical Memorandum NMFS‐SEFSC‐396.

10.1046/j.1365-294x.1998.00355.x

FitzSimmons NN, 1995, Conservation and dynamics of microsatellite loci over 300 million years of marine turtle evolution, Molecular Biology and Evolution, 12, 432

FitzSimmons NN, 1997, Philopatry of male marine turtles inferred from mitochondrial markers, Proceedings of the National Academy of Sciences, USA, 94, 8912, 10.1073/pnas.94.16.8912

10.1111/j.1558-5646.1994.tb01350.x

10.1016/S0001-6519(04)78497-6

Goudet J, 1994, fstat: A program for IBM PC and compatibles to calculate Weir and Cockerham's estimators of F‐statistics.

Hamann M, 2003, The Biology of Sea Turtles, 135

Harrison E, 2004, Report on the 2004 Green Turtle Program at Tortuguero, Costa Rica

10.1093/oxfordjournals.jhered.a110480

HiltermanML(2001) The sea turtles of Surinam 2000. WWF‐Guianas. Technical report GFECP11.

10.1038/sj.hdy.6800103

10.1016/S0022-0981(03)00118-7

10.1111/j.1469-185X.1999.tb00040.x

10.1006/anbe.1999.1248

10.1046/j.1471-8278.2001.00062.x

KalbH OwensD(1994)Differences between solitary and arribada nesting olive ridley females during the internesting period. In:Proceedings of the Fourteenth Annual Symposium on Sea Turtle Biology and Conservation(edsBjorndalKA BoltenAB JohnsonDA EliazarPJ) p.68. US Department of Commerce NOAA Technical Memorandum NMFS‐SEFSC‐351.

Kalb H, 1992, Proceedings of the 12th Annual Workshop on Sea Turtle Conservation and Biology, 57

10.1046/j.1365-294X.1999.00635.x

10.1073/pnas.0307982101

10.1071/WR9930513

Limpus CJ, 2003, Loggerhead Sea Turtles, 93

10.1038/355440a0

Márquez R, 1996, Olive ridley turtles (Lepidochelys olivacea) show signs of recovery at La Escobilla, Oaxaca, Marine Turtle Newsletter, 73, 5

Miller JD, 1997, The Biology of Sea Turtles, 51

10.1046/j.1365-294X.2002.01426.x

10.1111/j.1095-8649.2002.tb00908.x

10.1073/pnas.96.18.10236

10.1111/j.1471-8286.2004.00684.x

10.1038/hdy.1996.189

Pearse DE, 2001, Turtle mating systems: behavior, sperm storage, and genetic paternity, American Genetics Association, 92, 206

10.1007/s00265-001-0421-7

PlotkinPT(1994)Migratory and reproductive behaviour of the olive ridley turtle Lepidochelys olivacea(Eschscholtz 1829) in the eastern Pacific Ocean.PhD Thesis Texas A&M University College Station Texas.

Pritchard PCH, 1995, National Marine Fisheries Service and U.S. Fish and Wildlife Service Status Reviews of Sea Turtles Listed under the Endangered Species Act of 1973, 123

10.1093/oxfordjournals.jhered.a111573

10.1016/0169-5347(96)81045-7

10.1093/genetics/137.4.1147

Turtle Expert Working Group(1998)An Assessment of the Kemp's Ridley Turtle(Lepidochelys kempii)and Loggerhead(Caretta caretta)Sea Turtle Populations in the Western North Atlantic.NOAA Technical Memorandum NMFS‐SEFSC‐409.

Wagner HW, 1999, identity 1.0: Software for the analysis of microsatellite data.

Thông tin
Thông tin xuất bản

Molecular Ecology

Tập 15 Số 9

2567-2575

Thông tin tác giả