Microsatellite Marker Identification of a Triticum Aestivum -Aegilops Umbellulata Substitution Line with Powdery Mildew Resistance
Tóm tắt
Aegilops umbellulata acc. Y39 and Triticum carthlicum acc. PS5, immune to many powdery mildew isolates, were crossed to make an amphidiploid line Am9. The powdery mildew resistance of Am9 was transferred to common wheat cultivar Laizhou953 by crossing and backcrossing. In this study, the origin of powdery mildew resistance in a BC3F4:5 population derived from a cross of Am9 and Laizhou953 was identified. Microsatellite markers analysis showed that markers Xgwm257, Xgwm296, and Xgwm319, co-segregated with the powdery mildew resistance, whereas markers Xgwm210, Xgwm388/140, Xgwm388/170 and Xgwm526 were related to susceptibility and linked to resistance in repulsion. Of three markers related to resistance, Xgwm257 and Xgwm319 were codominant, whereas Xgwm296 was dominant. All three markers were Ae. umbellulata-specific indicating that resistance in the test population originated from Ae. umbellulata acc. Y39. The chromosome location and mapping of these linked microsatellite markers, the chromosome numbers of derived BC3F4:6 families, and chromosome pairing in F1 plants from a cross of a homozygous resistant BC3F4:5 plant and Laizhou953, showed that wheat chromosome 2B was substituted by Ae. umbellulata chromosome 2U. This is the first gene conferring powdery mildew resistance transferred to wheat from Ae. umbellulata, and it should be a novel resistance gene to powdery mildew. It was temporarily designated PmY39.
Tài liệu tham khảo
Aghaee-Sarbarzeh, M., Harjit-Singh & H.S. Dhaliwal, 2001. A microsatellite marker linked to leaf rust resistance transferred from Aegilops triuncialis into hexaploid wheat. Plant Breed 120: 259–261.
Chen, S.A., Y.C. Dong, S.J. Xu, R.H. Zhou, X.Q. Li & J.X. Wang, 1990. Gene mapping of resistance to powdery mildew in Triticum persicum-Aegilops tauschii amphidiloid Am3. Sci Agric Sin 23: 17–21.
Dograr, N., S. Akin-Yalin & M.S. Akkaya, 2000. Discriminating durum wheat cultivars using highly polymorphic simple sequence repeat DNA markers. Plant Breed 119: 360–362.
Duan, X.Y., B.Q. Sheng, Y.L. Zhou & Q.J. Xiang, 1998. Monitoring of the virulence population of Erysiphe graminis f. sp. tritici. Acta Phytophylac Sin 25: 31–36.
Duan, X.Y., Q.J. Xiang, Y.L. Zhou, B.Q. Sheng & Z.Y. Zhao, 2001. Allelic analysis of powdery mildew resistance genes in four Chinese landraces. Acta Phytopathol Sin 31(3, Suppl.): 32–35.
Fahima, T., M.S. Röder, A. Grama & E. Nevo, 1998. Microsatellite DNA polymorphism divergence in Triticum dicoccoides accessions highly resistant to yellow rust. Theor Appl Genet 96: 187–195.
Friebe, B., J. Jiang, N. Tuleen & B.S. Gill, 1995. Standard karyotype of Triticum umbellulatum and the characterization of derived chromosome addition and translocation lines in wheat wheat. Theor Appl Genet 90: 150–156.
Hammer, K., A.A. Filatenko & V. Korzun, 2000. Microsatellite markers – a new tool for distinguishing diploid wheat species. Genet Res Crop Evol 47: 497–505.
Hsam, S.L.K., I.F. Lapochkina & F.J. Zeller, 2003. Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.).8. Gene Pm32 in a wheat-Aegilops speltoides translocation line. Euphytica 133: 367–370.
Järvae, K., H.O. Peusha, J. Jtsymbalova, S. Tamm, K.M. Devos & T.M. Enno, 2000. Chromosomal location of a Triticum timopheevii-derived powdery mildew resistance gene transferred to common wheat. Genome 43: 377–381.
Lutz, J.E., P.B. Limpert & F.J. Zeller, 1992. Identification of powery mildew resistance genes in common wheat (Triticum aestivum L.) I. Czechoslovakian cultivars. Plant Breed 108: 33–39.
McIntosh, R.A., K.M. Devos, J. Dubcovsky, C.F. Morris & W.J. Rogers, 2003. Catalogue of gene symbols for wheat: 2003 Supplement. http://wheat.pw.usda.gov/
Michelmore, R.M., I. Paran & R.V. Kesseli, 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88: 9828–9832.
Peil, A., V. Korzun, V. Schubert, E. Schumann, W.E. Weber & M.S. Röder, 1998. The application of wheat microsatellites to identify disomic Triticum aestivum-Aegilops markgrafii addition lines. Theor Appl Genet 96: 138–146.
Röder, M.S., V. Korzun, K. Wendehake, J. Plaschke, M.H. Tixier, P. Leroy & M.W. Ganal, 1998. A microsatellite map of wheat. Genetics 149: 2007–2023.
Sharp, P.J, M. Kreis, P.R. Shewry & M.D. Gale, 1988. Location of β-amylase sequence in wheat and its relatives. Theor Appl Genet 75: 289–290.
Sourdille, P., M. Tavaud, G. Charmet & M. Bernard, 2001. Transferability of wheat microsatellites to diploid Triticeae species carrying the A, B and D genomes. Theor Appl Genet 103: 346–352.
Stephenson, P., G. Bryan, J. Kirby, A. Collins, K. Devos, C. Busso & M. Gale, 1998. Fifty new microsatellite loci for the wheat genetic map. Theor Appl Genet 97: 946–949.
Sun, G.L., B. Salomon & R.V. Bothmer, 1997. Analysis of tetraploid Elymus species using wheat microsatellite markers and RAPD markers. Genome 40: 806–814.
Tixier, M.H., P. Sourdille, M. Röder, P. Leroy & M. Bernard, 1997. Detection of wheat microsatellites using a non radioactive silver-nitrate staining method. J Genet & Breed 51: 175–177.
Xu, S.J., Y.C. Dong, S.A. Chen, R.H. Zhou, X.Q. Li & L.H. Li, 1990. Evaluation and utilization of the resistance of amphiploids between Triticum and Aegilops species to wheat diseases. Acta Agron Sin 16: 106–111.
Zhang, H., J. Jia, M.D. Gale & K.M. Devos, 1998. Relationships between the chromosomes of Aegilops umbellulata and wheat. Theor Appl Genet 96: 69–75.
Zhu, Z.D., R.H. Zhou, Y.C. Dong & J.Z. Jia, 2003. Analysis of powdery mildew resistance genes in some tetraploid wheat-Aegilops amphidiploids and their parents. J Plant Genet Res 4: 137–143.
Zhu, Z.D., R.H. Zhou, X.Y. Kong, Y.C. Dong & J.Z. Ji, 2005. Microsatellite markers linked to 2 powdery mildew resistance genes introgressed from Triticum carthlicum accession PS5 into common wheat. Genome 48: 585–590.