Microbioreactors for Bioprocess Development

SAGE Publications - Tập 12 Số 3 - Trang 143-151 - 2007
Zhiyu Zhiyu1, Gerardo Gerardo2, Paolo Paolo1, Anthony J. Anthony J.1, Oliver Oliver2, Klavs F. Klavs F.1
1Massachusetts Institute of Technology, Cambridge, MA
2Technical University of Denmark (DTU), Lyngby, Denmark

Tóm tắt

As a step toward high-throughput bioprocess development, we present design, fabrication, and characterization of polymer based microbioreactors integrated with automated sensors and actuators. The devices are realized, in increasing levels of complexity, in poly(dimethylsiloxane) and poly(methyl methacrylate) by micromachining and multilayer thermal compression bonding procedures. Online optical measurements for optical density, pH, and dissolved oxygen are integrated. Active mixing is made possible by a miniature magnetic stir bar. Plug-in-and-flow microfluidic connectors and fabricated polymer micro-optical lenses/connectors are integrated in the microbioreactors for fast set up and easy operation. Application examples demonstrate the feasibility of culturing microbial cells, specifically Escherichia coli, in 150 μL-volume bioreactors in batch, continuous, and fed-batch operations. (JALA 2007;12:143–51)

Tài liệu tham khảo

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SAGE Publications

Tập 12 Số 3

143-151

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